Bouw van een eigen ontwerp electrospray ionisatiebron voor een quadrupool massa-spectrometer en enkele toepassingen

2.50
Hdl Handle:
http://hdl.handle.net/10029/10082
Title:
Bouw van een eigen ontwerp electrospray ionisatiebron voor een quadrupool massa-spectrometer en enkele toepassingen
Authors:
Evers EAIM; Jong APJM de
Other Titles:
[Home-built electrospray ionisation source for a quadrupole mass-spectrometer.]
Abstract:
This report describes the manufacturing, the working and results of preliminary applications of a home-built electrospray ionisation source. The source was constructed for a quadrupole mass-spectrometer (MS) (Finnigan 4000). The elements of the source and interface are: a micro needle at high potential to the capillary, a capillary for transport of the ions and a sampling orifice (skimmer) to the mass analyser and detector. That interface enables the transport of ions from atmospheric pressure to the required high vacuum in the mass analyser as well as an appropriate focussing of ions corresponding. The design concerns the physical dimensions of the different parts and the electrical potentials. The results show that the electrospray process, the transport and the focussing operate satisfactorely. The highest sensitivity was measured for Gramicidin S (polypeptide, MW 1141 Dalton) of about 10 femtomol. For substances with a lower molecular weight the sensitivity was much lower and ranged from about 100 fmol for the polysaccharide streptomycin (MW 581 Da) to 50 pmol for polar low MW herbicides glyfosate and glufosinate. It seems useful to construct an extra focussing device between the skinner and the analyser to enhance the transmission efficiency for the low molecular weight species (<400 Da).
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
31-Mar-1994
URI:
http://hdl.handle.net/10029/10082
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/502501016.html
Language:
nl
Series/Report no.:
RIVM Rapport 502501016
Appears in Collections:
RIVM reports - old archive

Full metadata record

DC FieldValue Language
dc.contributor.authorEvers EAIMen_US
dc.contributor.authorJong APJM deen_US
dc.date.accessioned2007-03-09T16:18:34Z-
dc.date.available2007-03-09T16:18:34Z-
dc.date.issued1994-03-31en_US
dc.identifier502501016en_US
dc.identifier.urihttp://hdl.handle.net/10029/10082-
dc.description.abstractThis report describes the manufacturing, the working and results of preliminary applications of a home-built electrospray ionisation source. The source was constructed for a quadrupole mass-spectrometer (MS) (Finnigan 4000). The elements of the source and interface are: a micro needle at high potential to the capillary, a capillary for transport of the ions and a sampling orifice (skimmer) to the mass analyser and detector. That interface enables the transport of ions from atmospheric pressure to the required high vacuum in the mass analyser as well as an appropriate focussing of ions corresponding. The design concerns the physical dimensions of the different parts and the electrical potentials. The results show that the electrospray process, the transport and the focussing operate satisfactorely. The highest sensitivity was measured for Gramicidin S (polypeptide, MW 1141 Dalton) of about 10 femtomol. For substances with a lower molecular weight the sensitivity was much lower and ranged from about 100 fmol for the polysaccharide streptomycin (MW 581 Da) to 50 pmol for polar low MW herbicides glyfosate and glufosinate. It seems useful to construct an extra focussing device between the skinner and the analyser to enhance the transmission efficiency for the low molecular weight species (<400 Da).en
dc.format.extent894000 bytesen_US
dc.format.extent915288 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isonlen_US
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVMen_US
dc.relation.ispartofseriesRIVM Rapport 502501016en_US
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/502501016.htmlen_US
dc.subject.othermass spectrometryen
dc.subject.otherelectrospray ionisation sourceen
dc.subject.otherelectrospray ionisatie-bronen
dc.subject.othermassaspectrometrienl
dc.titleBouw van een eigen ontwerp electrospray ionisatiebron voor een quadrupool massa-spectrometer en enkele toepassingenen_US
dc.title.alternative[Home-built electrospray ionisation source for a quadrupole mass-spectrometer.]en_US
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