Analysis of Bordetella pertussis populations in European countries with different vaccination policies.

2.50
Hdl Handle:
http://hdl.handle.net/10029/10705
Title:
Analysis of Bordetella pertussis populations in European countries with different vaccination policies.
Authors:
Amersfoorth, S C M van; Schouls, L M; Heide, H G J van der; Advani, A; Hallander, H O; Bondeson, K; König, C H W von; Riffelmann, M; Vahrenholz, C; Guiso, N; Caro, V; Njamkepo, E; He, Q; Mertsola, J; Mooi, F R
Abstract:
Despite the widespread use of pertussis vaccines during the last decades, pertussis has remained an endemic disease with frequent epidemic outbreaks. Currently two types of vaccines are used: whole-cell vaccines (WCVs) and recently developed acellular vaccines (ACVs). The long-term aim of our studies is to assess the effect of different vaccination policies on the population structure of Bordetella pertussis and ultimately on the disease burden in Europe. In the present study, a total of 102 B. pertussis isolates from the period 1998 to 2001 from five European countries (Finland, Sweden, Germany, The Netherlands, and France) were characterized. The isolates were analyzed by typing based on variable number of tandem repeats (VNTR); by sequencing of polymorphic genes encoding the surface proteins pertussis toxin S1 and S3 subunits (ptxA and ptxC), pertactin (prn), and tracheal colonization factor (tcfA); and by fimbrial serotyping. The results reveal a relationship between geographic location and VNTR types, the frequency of the ptxC alleles, and serotypes. We have not observed a relationship between the strain characteristics we studied and vaccination programs. Our results provide a baseline which can be used to reveal changes in the B. pertussis population in Europe in the coming years.
Citation:
J. Clin. Microbiol. 2005, 43(6):2837-43
Issue Date:
1-Jun-2005
URI:
http://hdl.handle.net/10029/10705
DOI:
10.1128/JCM.43.6.2837-2843.2005
PubMed ID:
15956406
Type:
Article
Language:
en
ISSN:
0095-1137
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorAmersfoorth, S C M van-
dc.contributor.authorSchouls, L M-
dc.contributor.authorHeide, H G J van der-
dc.contributor.authorAdvani, A-
dc.contributor.authorHallander, H O-
dc.contributor.authorBondeson, K-
dc.contributor.authorKönig, C H W von-
dc.contributor.authorRiffelmann, M-
dc.contributor.authorVahrenholz, C-
dc.contributor.authorGuiso, N-
dc.contributor.authorCaro, V-
dc.contributor.authorNjamkepo, E-
dc.contributor.authorHe, Q-
dc.contributor.authorMertsola, J-
dc.contributor.authorMooi, F R-
dc.date.accessioned2007-03-20T10:11:14Z-
dc.date.available2007-03-20T10:11:14Z-
dc.date.issued2005-06-01-
dc.identifier.citationJ. Clin. Microbiol. 2005, 43(6):2837-43en
dc.identifier.issn0095-1137-
dc.identifier.pmid15956406-
dc.identifier.doi10.1128/JCM.43.6.2837-2843.2005-
dc.identifier.urihttp://hdl.handle.net/10029/10705-
dc.description.abstractDespite the widespread use of pertussis vaccines during the last decades, pertussis has remained an endemic disease with frequent epidemic outbreaks. Currently two types of vaccines are used: whole-cell vaccines (WCVs) and recently developed acellular vaccines (ACVs). The long-term aim of our studies is to assess the effect of different vaccination policies on the population structure of Bordetella pertussis and ultimately on the disease burden in Europe. In the present study, a total of 102 B. pertussis isolates from the period 1998 to 2001 from five European countries (Finland, Sweden, Germany, The Netherlands, and France) were characterized. The isolates were analyzed by typing based on variable number of tandem repeats (VNTR); by sequencing of polymorphic genes encoding the surface proteins pertussis toxin S1 and S3 subunits (ptxA and ptxC), pertactin (prn), and tracheal colonization factor (tcfA); and by fimbrial serotyping. The results reveal a relationship between geographic location and VNTR types, the frequency of the ptxC alleles, and serotypes. We have not observed a relationship between the strain characteristics we studied and vaccination programs. Our results provide a baseline which can be used to reveal changes in the B. pertussis population in Europe in the coming years.en
dc.format.extent130006 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleAnalysis of Bordetella pertussis populations in European countries with different vaccination policies.en
dc.typeArticleen
dc.format.digYES-
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