ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.

2.50
Hdl Handle:
http://hdl.handle.net/10029/10708
Title:
ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.
Authors:
Arend, Sandra M; Haas, Petra de; Leyten, Eliane; Rosenkrands, Ida; Rigouts, Leen; Andersen, Peter; Mijs, Wouter; Dissel, Jaap T van; Soolingen, Dick van
Abstract:
Mycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.
Citation:
J. Infect. Dis. 2005, 191(8):1301-10
Issue Date:
15-Apr-2005
URI:
http://hdl.handle.net/10029/10708
DOI:
10.1086/428950
PubMed ID:
15776377
Type:
Article
Language:
en
ISSN:
0022-1899
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorArend, Sandra M-
dc.contributor.authorHaas, Petra de-
dc.contributor.authorLeyten, Eliane-
dc.contributor.authorRosenkrands, Ida-
dc.contributor.authorRigouts, Leen-
dc.contributor.authorAndersen, Peter-
dc.contributor.authorMijs, Wouter-
dc.contributor.authorDissel, Jaap T van-
dc.contributor.authorSoolingen, Dick van-
dc.date.accessioned2007-03-20T13:24:59Z-
dc.date.available2007-03-20T13:24:59Z-
dc.date.issued2005-04-15-
dc.identifier.citationJ. Infect. Dis. 2005, 191(8):1301-10en
dc.identifier.issn0022-1899-
dc.identifier.pmid15776377-
dc.identifier.doi10.1086/428950-
dc.identifier.urihttp://hdl.handle.net/10029/10708-
dc.description.abstractMycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.en
dc.format.extent409173 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.en
dc.typeArticleen
dc.format.digYES-

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