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Webbased Archive of RIVM Publications > Articles and other publications by RIVM employees > Infectious Diseases > ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.


Please use this identifier to cite or link to this item: http://hdl.handle.net/10029/10708
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Title: ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.
Authors: Arend, Sandra M
Haas, Petra de
Leyten, Eliane
Rosenkrands, Ida
Rigouts, Leen
Andersen, Peter
Mijs, Wouter
Dissel, Jaap T van
Soolingen, Dick van
Citation: J. Infect. Dis. 2005, 191(8):1301-10
Issue Date: 15-Apr-2005
URI: http://hdl.handle.net/10029/10708
DOI: 10.1086/428950
PubMed ID: 15776377
Abstract: Mycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.
Type: Article
Language: en
ISSN: 0022-1899
Appears in Collections:  Infectious Diseases

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