Morphometric analysis of peroxisome proliferation by phthalate esters in rat liver

2.50
Hdl Handle:
http://hdl.handle.net/10029/255541
Title:
Morphometric analysis of peroxisome proliferation by phthalate esters in rat liver
Authors:
Dormans JAMA; Jansen EHJM; de Vlugt-van den Koedijk FM; Riool-Nesselaar G
Other Titles:
Morphometrische analyse van peroxisoom proliferatie door ftalaatesters in rattelever
Abstract:
Morphometric analysis was performed on liver sections of rats at light (LM) and electron microscopical (EM) level to demonstrate proliferation of peroxisomes after administration of di (2-ethylhexyl)phthalate at dietary levels of 0, 60, 200, 600, 2000 and 6000 mg/kg diet for 2 weeks. Enzyme histochemical demonstration of catalase was carried out to demonstrate the presence of peroxisomes. The results of the LM-morphometric analysis will be compared with those of the biochemical analysis, reported elsewhere. The LM-morphometric data will be tested by EM-morphometry in a limited number of groups. With the LM- as well as the EM-method the volume density of peroxisomes and the number of peroxisomes were significantly increased after administration of 200 mg DEHP/kg diet during 2 weeks. However, LM-morphometry of peroxisomes by enzyme histochemical demonstration of catalase is suitable to compare the capacity of various phthalate esters to induce proliferation of peroxisomes, realising that the measured values of volume density at LM-level are not true values, but relative values. With the catalase reaction, enzyme histochemical morphometry of peroxisomes showed a dose-without-effect of 60 mg DEHP/kg diet.<br>
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
30-Jun-1992
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/618902003.html
Type:
Onderzoeksrapport
Language:
en
Sponsors:
HIGB
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorDormans JAMA-
dc.contributor.authorJansen EHJM-
dc.contributor.authorde Vlugt-van den Koedijk FM-
dc.contributor.authorRiool-Nesselaar G-
dc.date.accessioned2014-01-17T13:27:06-
dc.date.issued1992-06-30-
dc.identifier618902003-
dc.description.abstractMorphometric analysis was performed on liver sections of rats at light (LM) and electron microscopical (EM) level to demonstrate proliferation of peroxisomes after administration of di (2-ethylhexyl)phthalate at dietary levels of 0, 60, 200, 600, 2000 and 6000 mg/kg diet for 2 weeks. Enzyme histochemical demonstration of catalase was carried out to demonstrate the presence of peroxisomes. The results of the LM-morphometric analysis will be compared with those of the biochemical analysis, reported elsewhere. The LM-morphometric data will be tested by EM-morphometry in a limited number of groups. With the LM- as well as the EM-method the volume density of peroxisomes and the number of peroxisomes were significantly increased after administration of 200 mg DEHP/kg diet during 2 weeks. However, LM-morphometry of peroxisomes by enzyme histochemical demonstration of catalase is suitable to compare the capacity of various phthalate esters to induce proliferation of peroxisomes, realising that the measured values of volume density at LM-level are not true values, but relative values. With the catalase reaction, enzyme histochemical morphometry of peroxisomes showed a dose-without-effect of 60 mg DEHP/kg diet.<br>en
dc.description.sponsorshipHIGB-
dc.format.extent19 p-
dc.language.isoen-
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM-
dc.relation.ispartofRIVM Rapport 618902003-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/618902003.html-
dc.subject07nl
dc.subjectperoxisomennl
dc.subjectlevernl
dc.subjectftalaatestersnl
dc.subjectanalysenl
dc.subjectmorfometrienl
dc.subjectdiermodelnl
dc.subjectmicrobodiesen
dc.subjectliveren
dc.subjectphthalate estersen
dc.subjectanalysisen
dc.subjectmorphometryen
dc.subjectanimal modelsen
dc.titleMorphometric analysis of peroxisome proliferation by phthalate esters in rat liveren
dc.title.alternativeMorphometrische analyse van peroxisoom proliferatie door ftalaatesters in rattelevernl
dc.typeOnderzoeksrapport-
dc.date.updated2014-01-17T12:29:36Z-
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