Identificatie en kwantificering van Escherichia coli door bepaling van de beta-glucuronidase-activiteit

2.50
Hdl Handle:
http://hdl.handle.net/10029/255788
Title:
Identificatie en kwantificering van Escherichia coli door bepaling van de beta-glucuronidase-activiteit
Authors:
Schets FM; Havelaar AH
Other Titles:
Identification and quantification of Escherichia coli by determination of beta-glucuronidase-activity
Abstract:
The determination of E.coli as a fecal indicator for surface- and drinking water according to NEN 6261 is based on the ability of E.coli to produce indole from tryptophane. E.coli is also able to use the constitutive enzyme beta-glucuronidase to produce the fluorescent methylum-belliferyl from the substrate 4-methylumbelliferyl-beta-D- glucuronide (MUG). This report describes research on the possibility to use the beta-glucuronidase-activity as an identification- characteristic for E.coli. 14% of the isolated E.coli strains was found to be beta-glucuronidase-negative at 44 degr. C. 24% of these strains showed a positive beta-glucuronidase-reaction at 37 degr. C. Two percent of the isolated E.coli strains was indole-negative and four percent of strains other than E.coli gave false positive reactions in this test. There were no false-positive beta-glucuronidase reactions. It is concluded that the indole-reaction gives better results than the beta-glucuronidase-reaction. The interpretation of the beta- glucuronidase- reaction is more difficult and it gives more false- negative results.<br>
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
30-Sep-1990
URI:
http://hdl.handle.net/10029/255788
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/148608001.html
Type:
Onderzoeksrapport
Language:
nl
Sponsors:
RIVM
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorSchets FM-
dc.contributor.authorHavelaar AH-
dc.date.accessioned2012-12-12T12:29:42Z-
dc.date.available2012-12-12T12:29:42Z-
dc.date.issued1990-09-30-
dc.identifier148608001-
dc.identifier.urihttp://hdl.handle.net/10029/255788-
dc.description.abstractThe determination of E.coli as a fecal indicator for surface- and drinking water according to NEN 6261 is based on the ability of E.coli to produce indole from tryptophane. E.coli is also able to use the constitutive enzyme beta-glucuronidase to produce the fluorescent methylum-belliferyl from the substrate 4-methylumbelliferyl-beta-D- glucuronide (MUG). This report describes research on the possibility to use the beta-glucuronidase-activity as an identification- characteristic for E.coli. 14% of the isolated E.coli strains was found to be beta-glucuronidase-negative at 44 degr. C. 24% of these strains showed a positive beta-glucuronidase-reaction at 37 degr. C. Two percent of the isolated E.coli strains was indole-negative and four percent of strains other than E.coli gave false positive reactions in this test. There were no false-positive beta-glucuronidase reactions. It is concluded that the indole-reaction gives better results than the beta-glucuronidase-reaction. The interpretation of the beta- glucuronidase- reaction is more difficult and it gives more false- negative results.<br>en
dc.description.sponsorshipRIVM-
dc.format.extent31 p-
dc.language.isonl-
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM-
dc.relation.ispartofRIVM Rapport 148608001-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/148608001.html-
dc.subject01nl
dc.subjecte.colinl
dc.subjectbeta-glucuronidasenl
dc.subjectmugnl
dc.titleIdentificatie en kwantificering van Escherichia coli door bepaling van de beta-glucuronidase-activiteitnl
dc.title.alternativeIdentification and quantification of Escherichia coli by determination of beta-glucuronidase-activityen
dc.typeOnderzoeksrapport-
dc.date.updated2012-12-12T12:29:43Z-
All Items in WARP are protected by copyright, with all rights reserved, unless otherwise indicated.