Monomer mapping: A PC-based software tool facilitating the determination of monomer compositions from mass spectrometric molecular weight data

2.50
Hdl Handle:
http://hdl.handle.net/10029/256019
Title:
Monomer mapping: A PC-based software tool facilitating the determination of monomer compositions from mass spectrometric molecular weight data
Authors:
Setten DC van; Werken G van de
Other Titles:
[Monomer mapping: Een PC-programma voor de bepaling van monomeersamenstellingen op grond van massaspectrometrisch verkregen molecuulgewichten.]
Abstract:
Abstract niet beschikbaar

A method to establish the monomer composition of (bio) macromolecules has been developed. The method, to be called monomer mapping, involves the mass spectrometric determination of the molecular weight and a computer program. The program generates monomer compositions corresponding to the measured molecular weight. If more than one monomer composition for the polymer is generated by monomer mapping, a further limitation can be obtained from the determination of the number of exchangeable hydrogens. Monomer mapping can be used to determine the monomer compositions of individual components in mixtures without applying (chromatographic) separation techniques. This is a clear advantage over methods involving monomerization and consecutive determination of the relative amount of monomers. As a limitation, monomer mapping requires the elemental compositions of all monomer types possibly present in the polymer. The largest molecular weight resulting into one monomer composition depends on the number of monomer types considered. The method can be used as a supplement to amino acid analysis, generally resulting into the actual amina acid composition of peptides consisting of up to 25 amino acids. Monomer mapping was successfully applied to determine glycosyl compositions of fifty saponins.
Affiliation:
LOC
Issue Date:
30-Apr-1994
URI:
http://hdl.handle.net/10029/256019
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/343605002.html
Type:
Onderzoeksrapport
Language:
en
Sponsors:
RIVM
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorSetten DC van-
dc.contributor.authorWerken G van de-
dc.date.accessioned2012-12-12T13:02:53Z-
dc.date.available2012-12-12T13:02:53Z-
dc.date.issued1994-04-30-
dc.identifier343605002-
dc.identifier.urihttp://hdl.handle.net/10029/256019-
dc.description.abstractAbstract niet beschikbaarnl
dc.description.abstractA method to establish the monomer composition of (bio) macromolecules has been developed. The method, to be called monomer mapping, involves the mass spectrometric determination of the molecular weight and a computer program. The program generates monomer compositions corresponding to the measured molecular weight. If more than one monomer composition for the polymer is generated by monomer mapping, a further limitation can be obtained from the determination of the number of exchangeable hydrogens. Monomer mapping can be used to determine the monomer compositions of individual components in mixtures without applying (chromatographic) separation techniques. This is a clear advantage over methods involving monomerization and consecutive determination of the relative amount of monomers. As a limitation, monomer mapping requires the elemental compositions of all monomer types possibly present in the polymer. The largest molecular weight resulting into one monomer composition depends on the number of monomer types considered. The method can be used as a supplement to amino acid analysis, generally resulting into the actual amina acid composition of peptides consisting of up to 25 amino acids. Monomer mapping was successfully applied to determine glycosyl compositions of fifty saponins.en
dc.description.sponsorshipRIVM-
dc.format.extent36 p-
dc.language.isoen-
dc.relation.ispartofRIVM Rapport 343605002-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/343605002.html-
dc.subject10nl
dc.subjectanalysemethodenl
dc.subjectmengselnl
dc.subjectsoftwarenl
dc.subjectmonomerennl
dc.subjectanalytical methodsen
dc.subjectmixturesen
dc.subjectsoftwareen
dc.subjectmonomersen
dc.titleMonomer mapping: A PC-based software tool facilitating the determination of monomer compositions from mass spectrometric molecular weight dataen
dc.title.alternative[Monomer mapping: Een PC-programma voor de bepaling van monomeersamenstellingen op grond van massaspectrometrisch verkregen molecuulgewichten.]nl
dc.typeOnderzoeksrapport-
dc.contributor.departmentLOC-
dc.date.updated2012-12-12T13:02:53Z-
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