Onderzoek naar de mogelijkheden om in vitro antistof produktie door immune cellen te gebruiken voor de controle van difterie en tetanus bevattende vaccins

2.50
Hdl Handle:
http://hdl.handle.net/10029/256357
Title:
Onderzoek naar de mogelijkheden om in vitro antistof produktie door immune cellen te gebruiken voor de controle van difterie en tetanus bevattende vaccins
Authors:
Loggen HG; Akkermans AM; van de Donk HJM; Kreeftenberg JG; Hendriksen CFM; de Jong WH
Other Titles:
Research of the possibilities to use in vitro antibody production by immune cells for the control of diphtheria and tetanus containing vaccines
Abstract:
This report describes the possible use of an in vitro culture system for the production of antibodies, as testsystem for the control of diphtheria and tetanus containing vaccines like DPTP (Diphtheria, Pertussis, Tetanus and Polio) and DTP (Diphtheria, Tetanus and Polio). Both in a human and rabbit testsystem an increase was found in the number of antibody secreting cells, after incubation of PBL's (peripheral blood lymphocytes) of immunized donors with DPTP or DTP vaccine. In both test systems antibodies against diphtheria and tetanus were observed. The antibody production in the human system, however was based on a false positive assay. The antibodies, added to initiate antibody production in the immune cells, were passively transferred from the stimulation phase of the assay day 1 to day 4, to the production phase of the assay day 5 to day 12. The passive transfer was caused by the presence of the insoluble aluminium phosphate used as adjuvant in the vaccines. Both for diphtheria and tetanus antigen specific antibody production was observed in the rabbit culture system. For the tetanus antigen clearly a dose response relationship was present, which was not found for the diphtheria antigen. It was technically not possble to obtain a reproducible harvest of the rabbit PBL's, which limits the possibilities of this system for vaccine control. Conclusively we can say that the control of diphtheria and tetanus vaccine by means of in vitro antibody production is not (yet) possible. Further research is needed to optimize and standardize the in vitro testsystems before they can be used for routine vaccine control.<br>
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
31-Dec-1992
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/458802001.html
Type:
Onderzoeksrapport
Language:
nl
Sponsors:
VHI
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorLoggen HG-
dc.contributor.authorAkkermans AM-
dc.contributor.authorvan de Donk HJM-
dc.contributor.authorKreeftenberg JG-
dc.contributor.authorHendriksen CFM-
dc.contributor.authorde Jong WH-
dc.date.accessioned2014-01-17T13:27:35-
dc.date.issued1992-12-31-
dc.identifier458802001-
dc.description.abstractThis report describes the possible use of an in vitro culture system for the production of antibodies, as testsystem for the control of diphtheria and tetanus containing vaccines like DPTP (Diphtheria, Pertussis, Tetanus and Polio) and DTP (Diphtheria, Tetanus and Polio). Both in a human and rabbit testsystem an increase was found in the number of antibody secreting cells, after incubation of PBL's (peripheral blood lymphocytes) of immunized donors with DPTP or DTP vaccine. In both test systems antibodies against diphtheria and tetanus were observed. The antibody production in the human system, however was based on a false positive assay. The antibodies, added to initiate antibody production in the immune cells, were passively transferred from the stimulation phase of the assay day 1 to day 4, to the production phase of the assay day 5 to day 12. The passive transfer was caused by the presence of the insoluble aluminium phosphate used as adjuvant in the vaccines. Both for diphtheria and tetanus antigen specific antibody production was observed in the rabbit culture system. For the tetanus antigen clearly a dose response relationship was present, which was not found for the diphtheria antigen. It was technically not possble to obtain a reproducible harvest of the rabbit PBL's, which limits the possibilities of this system for vaccine control. Conclusively we can say that the control of diphtheria and tetanus vaccine by means of in vitro antibody production is not (yet) possible. Further research is needed to optimize and standardize the in vitro testsystems before they can be used for routine vaccine control.<br>en
dc.description.sponsorshipVHI-
dc.format.extent41 p-
dc.language.isonl-
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM-
dc.relation.ispartofRIVM Rapport 458802001-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/458802001.html-
dc.subject02nl
dc.subject92-4nl
dc.subjectin vitronl
dc.subjectdifterienl
dc.subjecttetanusnl
dc.subjectperifere bloed lymfocytennl
dc.subjectvaccinsnl
dc.subjectperipheral blood lymphocytesnl
dc.subjectdptp vaccinenl
dc.subjectdiphtheria toxoiden
dc.subjecttetanus toxoiden
dc.subjectdpt vaccineen
dc.subjectquality controlen
dc.subjectantibodiesen
dc.subjectin vitro methodsen
dc.titleOnderzoek naar de mogelijkheden om in vitro antistof produktie door immune cellen te gebruiken voor de controle van difterie en tetanus bevattende vaccinsnl
dc.title.alternativeResearch of the possibilities to use in vitro antibody production by immune cells for the control of diphtheria and tetanus containing vaccinesen
dc.typeOnderzoeksrapport-
dc.date.updated2014-01-17T12:30:06Z-
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