Onderzoek naar de bruikbaarheid van electrospray ionisatie-massaspectrometrie voor de bepaling van de molecuulmassa van kleine eiwitten

2.50
Hdl Handle:
http://hdl.handle.net/10029/256694
Title:
Onderzoek naar de bruikbaarheid van electrospray ionisatie-massaspectrometrie voor de bepaling van de molecuulmassa van kleine eiwitten
Authors:
Heeft E van der; Jong APJM de
Other Titles:
[Investigation into the utility of electrospray ionization-mass spectrometry for the determination of the molecular mass of small proteins.]
Abstract:
Abstract niet beschikbaar

This report describes the results of an investigation into the utility of electrospray ionization - mass spectrometry (ESI-MS) for the determination of the molecular mass of small proteins (up to 17 kDa). The molecular mass of four pure proteins, was determined by ESI-MS. From the known molecular mass of the proteins, the accuracy of the method can be estimated. Two methods were applied to calculate the molecular mass from the acquired ESI mass spectra. In terms of accuracy and precision of the measured molecular mass, the two methods were almost equal. The results of this investigation show that an accuracy of about 0.01% and precision of 0.006% can be achieved. The acquisition of the ESI mass spectro required 10 pmol of protein.
Issue Date:
31-Oct-1993
URI:
http://hdl.handle.net/10029/256694
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/502501011.html
Type:
Onderzoeksrapport
Language:
nl
Sponsors:
RIVM
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorHeeft E van der-
dc.contributor.authorJong APJM de-
dc.date.accessioned2012-12-12T14:27:18Z-
dc.date.available2012-12-12T14:27:18Z-
dc.date.issued1993-10-31-
dc.identifier502501011-
dc.identifier.urihttp://hdl.handle.net/10029/256694-
dc.description.abstractAbstract niet beschikbaarnl
dc.description.abstractThis report describes the results of an investigation into the utility of electrospray ionization - mass spectrometry (ESI-MS) for the determination of the molecular mass of small proteins (up to 17 kDa). The molecular mass of four pure proteins, was determined by ESI-MS. From the known molecular mass of the proteins, the accuracy of the method can be estimated. Two methods were applied to calculate the molecular mass from the acquired ESI mass spectra. In terms of accuracy and precision of the measured molecular mass, the two methods were almost equal. The results of this investigation show that an accuracy of about 0.01% and precision of 0.006% can be achieved. The acquisition of the ESI mass spectro required 10 pmol of protein.en
dc.description.sponsorshipRIVM-
dc.format.extent18 p-
dc.language.isonl-
dc.relation.ispartofRIVM Rapport 502501011-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/502501011.html-
dc.subject18nl
dc.subjecteiwittennl
dc.subjectmassa spectrometrienl
dc.subjectproteinsen
dc.subjectmass spectrometryen
dc.subjectmolecular weighten
dc.subjectmolecuulmassaen
dc.titleOnderzoek naar de bruikbaarheid van electrospray ionisatie-massaspectrometrie voor de bepaling van de molecuulmassa van kleine eiwittennl
dc.title.alternative[Investigation into the utility of electrospray ionization-mass spectrometry for the determination of the molecular mass of small proteins.]en
dc.typeOnderzoeksrapport-
dc.date.updated2012-12-12T14:27:18Z-
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