Biomonitoring of nitrate exposure. I. Determination of N-methylnicotinamide with capillary zone electrophoresis

2.50
Hdl Handle:
http://hdl.handle.net/10029/257581
Title:
Biomonitoring of nitrate exposure. I. Determination of N-methylnicotinamide with capillary zone electrophoresis
Authors:
de Fluiter P; Jansen EHJM; Zeilmaker MJ; Zomer G
Other Titles:
[Biomonitoring van blootstelling aan nitraat. I. Bepaling van N-methylnicotinamide met capillaire zone elektroforese.]
Abstract:
In the present study a method is developed for the quantitation of N-methylnicotinamide (NMN) in rat and human urine with capillary zone electrophoresis (CZE). Because NMN is considered as a possible biomarker for nitrite exposure, a quantitative method of analysis is required for further evaluation studies. In the present report CZE was evaluated as method of analysis for NMN. CZE turned out to be very suitable. No pretreatment of the human urine sample was needed before analysis and large series of samples can be handled. Although only a few human urine samples have been analysed at present, the CZE method looks very promising. In addition NMN concentrations in rat urine samples from a toxicity experiment have been determined. At this point it is questionable whether a dose-response relationship exists in the low concentration exposure range because the statistical significance disappeared after correction for the produced urine volume or creatinin content.<br>
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
31-Jul-1993
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/623830001.html
Type:
Onderzoeksrapport
Language:
en
Sponsors:
GHI
Appears in Collections:
RIVM official reports

Full metadata record

DC FieldValue Language
dc.contributor.authorde Fluiter P-
dc.contributor.authorJansen EHJM-
dc.contributor.authorZeilmaker MJ-
dc.contributor.authorZomer G-
dc.date.accessioned2014-01-17T13:18:14-
dc.date.issued1993-07-31-
dc.identifier623830001-
dc.description.abstractIn the present study a method is developed for the quantitation of N-methylnicotinamide (NMN) in rat and human urine with capillary zone electrophoresis (CZE). Because NMN is considered as a possible biomarker for nitrite exposure, a quantitative method of analysis is required for further evaluation studies. In the present report CZE was evaluated as method of analysis for NMN. CZE turned out to be very suitable. No pretreatment of the human urine sample was needed before analysis and large series of samples can be handled. Although only a few human urine samples have been analysed at present, the CZE method looks very promising. In addition NMN concentrations in rat urine samples from a toxicity experiment have been determined. At this point it is questionable whether a dose-response relationship exists in the low concentration exposure range because the statistical significance disappeared after correction for the produced urine volume or creatinin content.<br>en
dc.description.sponsorshipGHI-
dc.format.extent22 p-
dc.language.isoen-
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVM-
dc.relation.ispartofRIVM Rapport 623830001-
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/623830001.html-
dc.subject18nl
dc.subjectnitraatnl
dc.subjectblootstellingnl
dc.subjectbiomonitoringnl
dc.subjectanalysenl
dc.subjecturinenl
dc.subjectelectroforesenl
dc.subjectnitratesen
dc.subjectexposureen
dc.subjectbiological monitoringen
dc.subjectanalysisen
dc.subjecturineen
dc.subjectelectrophoresisen
dc.subjectn-methylnicotinamideen
dc.subjectcapillary zone electrophoresisen
dc.titleBiomonitoring of nitrate exposure. I. Determination of N-methylnicotinamide with capillary zone electrophoresisen
dc.title.alternative[Biomonitoring van blootstelling aan nitraat. I. Bepaling van N-methylnicotinamide met capillaire zone elektroforese.]nl
dc.typeOnderzoeksrapport-
dc.date.updated2014-01-17T12:20:44Z-
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