Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.

2.50
Hdl Handle:
http://hdl.handle.net/10029/4909
Title:
Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.
Authors:
Huard, Richard C; Fabre, Michel; Haas, Petra de; Lazzarini, Luiz Claudio Oliveira; Soolingen, Dick van; Cousins, Debby; Ho, John L
Abstract:
In a previous report, we described a PCR protocol for the differentiation of the various species of the Mycobacterium tuberculosis complex (MTC) on the basis of genomic deletions (R. C. Huard, L. C. de Oliveira Lazzarini, W. R. Butler, D. van Soolingen, and J. L. Ho, J. Clin. Microbiol. 41:1637-1650, 2003). That report also provided a broad cross-comparison of several previously identified, phylogenetically relevant, long-sequence and single-nucleotide polymorphisms (LSPs and SNPs, respectively). In the present companion report, we expand upon the previous work (i) by continuing the evaluation of known MTC phylogenetic markers in a larger collection of tubercle bacilli (n = 125), (ii) by evaluating additional recently reported MTC species-specific and interspecific polymorphisms, and (iii) by describing the identification and distribution of a number of novel LSPs and SNPs. Notably, new genomic deletions were found in various Mycobacterium tuberculosis strains, new species-specific SNPs were identified for "Mycobacterium canettii," Mycobacterium microti, and Mycobacterium pinnipedii, and, for the first time, intraspecific single-nucleotide DNA differences were discovered for the dassie bacillus, the oryx bacillus, and the two Mycobacterium africanum subtype I variants. Surprisingly, coincident polymorphisms linked one M. africanum subtype I genotype with the dassie bacillus and M. microti with M. pinnipedii, thereby suggesting closer evolutionary ties within each pair of species than had been previously thought. Overall, the presented data add to the genetic definitions of several MTC organisms as well as fine-tune current models for the evolutionary history of the MTC.
Citation:
J. Bacteriol. 2006, 188(12):4271-87
Issue Date:
1-Jun-2006
URI:
http://hdl.handle.net/10029/4909
DOI:
10.1128/JB.01783-05
PubMed ID:
16740934
Type:
Article
Language:
en
ISSN:
0021-9193
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorHuard, Richard C-
dc.contributor.authorFabre, Michel-
dc.contributor.authorHaas, Petra de-
dc.contributor.authorLazzarini, Luiz Claudio Oliveira-
dc.contributor.authorSoolingen, Dick van-
dc.contributor.authorCousins, Debby-
dc.contributor.authorHo, John L-
dc.date.accessioned2006-10-06T08:44:35Z-
dc.date.available2006-10-06T08:44:35Z-
dc.date.issued2006-06-01-
dc.identifier.citationJ. Bacteriol. 2006, 188(12):4271-87en
dc.identifier.issn0021-9193-
dc.identifier.pmid16740934-
dc.identifier.doi10.1128/JB.01783-05-
dc.identifier.urihttp://hdl.handle.net/10029/4909-
dc.description.abstractIn a previous report, we described a PCR protocol for the differentiation of the various species of the Mycobacterium tuberculosis complex (MTC) on the basis of genomic deletions (R. C. Huard, L. C. de Oliveira Lazzarini, W. R. Butler, D. van Soolingen, and J. L. Ho, J. Clin. Microbiol. 41:1637-1650, 2003). That report also provided a broad cross-comparison of several previously identified, phylogenetically relevant, long-sequence and single-nucleotide polymorphisms (LSPs and SNPs, respectively). In the present companion report, we expand upon the previous work (i) by continuing the evaluation of known MTC phylogenetic markers in a larger collection of tubercle bacilli (n = 125), (ii) by evaluating additional recently reported MTC species-specific and interspecific polymorphisms, and (iii) by describing the identification and distribution of a number of novel LSPs and SNPs. Notably, new genomic deletions were found in various Mycobacterium tuberculosis strains, new species-specific SNPs were identified for "Mycobacterium canettii," Mycobacterium microti, and Mycobacterium pinnipedii, and, for the first time, intraspecific single-nucleotide DNA differences were discovered for the dassie bacillus, the oryx bacillus, and the two Mycobacterium africanum subtype I variants. Surprisingly, coincident polymorphisms linked one M. africanum subtype I genotype with the dassie bacillus and M. microti with M. pinnipedii, thereby suggesting closer evolutionary ties within each pair of species than had been previously thought. Overall, the presented data add to the genetic definitions of several MTC organisms as well as fine-tune current models for the evolutionary history of the MTC.en
dc.format.extent212214 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleNovel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.en
dc.typeArticleen
dc.format.digYES-

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