Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis.

2.50
Hdl Handle:
http://hdl.handle.net/10029/621341
Title:
Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis.
Authors:
Xu, J; Bai, X; Wang, L B; Shi, H N; van der Giessen, J W B; Boireau, P; Liu, M Y; Liu, X L
Abstract:
Nematodes of the genus Trichinella are one of the most widespread zoonotic pathogens on the world, and they can still cause major public health problems in many parts of the world. Vaccination against the helminth nematode Trichinella could be a good strategy to reduce the risk of human and animal infection. It was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rTs-Serpin antigen. In this study, BALB/c mice were vaccinated by an intramuscular route with rTs-Serpin antigen from the parasite Trichinella spiralis in combination with three different adjuvant formulations: Montanide ISA201, Montanide IMS 1313 N PR VG and Freund's complete adjuvant/Freund's incomplete adjuvant (FCA/FIA). The dynamics of IgG, IgM, IgE and cytokine production from spleen cells and worm reduction rate of the vaccinated mice were analysed. The results showed that rTs-serpin can induce partial protection against Trichinella larvae challenge in mice, when compared to the FCA-/FIA-formulated vaccination, the IMS1313 plus rTs-serpin mixture showed higher humoral immunity and similar levels of cellular immunity and worm reduction rate. The study suggested that Montanide IMS nanoparticles 1313 are as effective as FCA but less toxic; thus, Montanide IMS nanoparticles 1313 can be used as a good candidate of adjuvant for developing vaccine against Trichinella spiralis.
Citation:
Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis. 2017, 39 (7) Parasite Immunol.
Journal:
Parasit Immunol 2017; 39(7):e12437
Issue Date:
Jul-2017
URI:
http://hdl.handle.net/10029/621341
DOI:
10.1111/pim.12437
PubMed ID:
28445612
Type:
Article
Language:
en
ISSN:
1365-3024
Appears in Collections:
Miscellaneous

Full metadata record

DC FieldValue Language
dc.contributor.authorXu, Jen
dc.contributor.authorBai, Xen
dc.contributor.authorWang, L Ben
dc.contributor.authorShi, H Nen
dc.contributor.authorvan der Giessen, J W Ben
dc.contributor.authorBoireau, Pen
dc.contributor.authorLiu, M Yen
dc.contributor.authorLiu, X Len
dc.date.accessioned2018-02-07T07:57:38Z-
dc.date.available2018-02-07T07:57:38Z-
dc.date.issued2017-07-
dc.identifier.citationInfluence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis. 2017, 39 (7) Parasite Immunol.en
dc.identifier.issn1365-3024-
dc.identifier.pmid28445612-
dc.identifier.doi10.1111/pim.12437-
dc.identifier.urihttp://hdl.handle.net/10029/621341-
dc.description.abstractNematodes of the genus Trichinella are one of the most widespread zoonotic pathogens on the world, and they can still cause major public health problems in many parts of the world. Vaccination against the helminth nematode Trichinella could be a good strategy to reduce the risk of human and animal infection. It was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rTs-Serpin antigen. In this study, BALB/c mice were vaccinated by an intramuscular route with rTs-Serpin antigen from the parasite Trichinella spiralis in combination with three different adjuvant formulations: Montanide ISA201, Montanide IMS 1313 N PR VG and Freund's complete adjuvant/Freund's incomplete adjuvant (FCA/FIA). The dynamics of IgG, IgM, IgE and cytokine production from spleen cells and worm reduction rate of the vaccinated mice were analysed. The results showed that rTs-serpin can induce partial protection against Trichinella larvae challenge in mice, when compared to the FCA-/FIA-formulated vaccination, the IMS1313 plus rTs-serpin mixture showed higher humoral immunity and similar levels of cellular immunity and worm reduction rate. The study suggested that Montanide IMS nanoparticles 1313 are as effective as FCA but less toxic; thus, Montanide IMS nanoparticles 1313 can be used as a good candidate of adjuvant for developing vaccine against Trichinella spiralis.en
dc.language.isoenen
dc.rightsArchived with thanks to Parasite immunologyen
dc.subject.meshAdjuvants, Immunologic-
dc.subject.meshAnimals-
dc.subject.meshAntigens, Helminth-
dc.subject.meshFemale-
dc.subject.meshFreund's Adjuvant-
dc.subject.meshImmunity, Humoral-
dc.subject.meshImmunization-
dc.subject.meshLarva-
dc.subject.meshLipids-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshRecombinant Proteins-
dc.subject.meshSerpins-
dc.subject.meshTrichinella spiralis-
dc.subject.meshTrichinellosis-
dc.titleInfluence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis.en
dc.typeArticleen
dc.identifier.journalParasit Immunol 2017; 39(7):e12437en

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