2.50
Hdl Handle:
http://hdl.handle.net/10029/6919
Title:
Establishment of a genotyping scheme for Coxiella burnetii.
Authors:
Svraka, Sanela; Toman, Rudolf; Skultety, Ludovit; Slaba, Katarina; Homan, Wieger L
Abstract:
Coxiella burnetii is the causative agent of Q fever. The bacterium is highly infectious and is classified as a category B biological weapon. The tools of molecular biology are of utmost importance in a rapid and unambiguous identification of C. burnetii in naturally occurring Q fever outbreaks, or in cases of a deliberate release of the infectious agent. In this work, development of a multiple locus variable number tandem repeats (VNTR) analysis (MLVA) for the characterization of C. burnetii is described. Sixteen C. burnetii isolates and five passage history/laboratory variants were characterized. The VNTR markers revealed many polymorphisms resulting in nine unique MLVA types that cluster into five different clusters. This proves that the MLVA system is highly discriminatory. The selected VNTR markers were stable. The MLVA method developed in this report is a promising tool for the characterization of C. burnetii isolates and their epidemiological study.
Citation:
FEMS Microbiol. Lett. 2006, 254(2):268-74
Issue Date:
1-Jan-2006
URI:
http://hdl.handle.net/10029/6919
DOI:
10.1111/j.1574-6968.2005.00036.x
PubMed ID:
16445755
Type:
Article
Language:
en
ISSN:
0378-1097
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorSvraka, Sanela-
dc.contributor.authorToman, Rudolf-
dc.contributor.authorSkultety, Ludovit-
dc.contributor.authorSlaba, Katarina-
dc.contributor.authorHoman, Wieger L-
dc.date.accessioned2007-01-05T09:00:32Z-
dc.date.available2007-01-05T09:00:32Z-
dc.date.issued2006-01-01-
dc.identifier.citationFEMS Microbiol. Lett. 2006, 254(2):268-74en
dc.identifier.issn0378-1097-
dc.identifier.pmid16445755-
dc.identifier.doi10.1111/j.1574-6968.2005.00036.x-
dc.identifier.urihttp://hdl.handle.net/10029/6919-
dc.description.abstractCoxiella burnetii is the causative agent of Q fever. The bacterium is highly infectious and is classified as a category B biological weapon. The tools of molecular biology are of utmost importance in a rapid and unambiguous identification of C. burnetii in naturally occurring Q fever outbreaks, or in cases of a deliberate release of the infectious agent. In this work, development of a multiple locus variable number tandem repeats (VNTR) analysis (MLVA) for the characterization of C. burnetii is described. Sixteen C. burnetii isolates and five passage history/laboratory variants were characterized. The VNTR markers revealed many polymorphisms resulting in nine unique MLVA types that cluster into five different clusters. This proves that the MLVA system is highly discriminatory. The selected VNTR markers were stable. The MLVA method developed in this report is a promising tool for the characterization of C. burnetii isolates and their epidemiological study.en
dc.format.extent152227 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleEstablishment of a genotyping scheme for Coxiella burnetii.en
dc.typeArticleen
dc.format.digYES-

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