Molecular evidence for association of chlamydiales bacteria with epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer).

2.50
Hdl Handle:
http://hdl.handle.net/10029/6921
Title:
Molecular evidence for association of chlamydiales bacteria with epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer).
Authors:
Meijer, Adam; Roholl, Paul J M; Ossewaarde, Jacobus M; Jones, Brian; Nowak, Barbara F
Abstract:
Epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer), previously associated with chlamydial bacterial infection using ultrastructural analysis, was further investigated by using molecular and immunocytochemical methods. Morphologically, all three species showed epitheliocystis cysts in the gills, and barramundi also showed lymphocystis cysts in the skin. From gill cysts of all three species and from skin cysts of barramundi 16S rRNA gene fragments were amplified by PCR and sequenced, which clustered by phylogenetic analysis together with other chlamydia-like organisms in the order Chlamydiales in a lineage separate from the family Chlamydiaceae. By using in situ RNA hybridization, 16S rRNA Chlamydiales-specific sequences were detected in gill cysts of silver perch and in gill and skin cysts of barramundi. By applying immunocytochemistry, chlamydial antigens (lipopolysaccharide and/or membrane protein) were detected in gill cysts of leafy seadragon and in gill and skin cysts of barramundi, but not in gill cysts of silver perch. In conclusion, this is the first time epitheliocystis agents of leafy seadragon, silver perch and barramundi have been undoubtedly identified as belonging to bacteria of the order Chlamydiales by molecular methods. In addition, the results suggested that lymphocystis cysts, known to be caused by iridovirus infection, could be coinfected with the epitheliocystis agent.
Citation:
Appl. Environ. Microbiol. 2006, 72(1):284-90
Issue Date:
1-Jan-2006
URI:
http://hdl.handle.net/10029/6921
DOI:
10.1128/AEM.72.1.284-290.2006
PubMed ID:
16391055
Type:
Article
Language:
en
ISSN:
0099-2240
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorMeijer, Adam-
dc.contributor.authorRoholl, Paul J M-
dc.contributor.authorOssewaarde, Jacobus M-
dc.contributor.authorJones, Brian-
dc.contributor.authorNowak, Barbara F-
dc.date.accessioned2007-01-05T09:28:24Z-
dc.date.available2007-01-05T09:28:24Z-
dc.date.issued2006-01-01-
dc.identifier.citationAppl. Environ. Microbiol. 2006, 72(1):284-90en
dc.identifier.issn0099-2240-
dc.identifier.pmid16391055-
dc.identifier.doi10.1128/AEM.72.1.284-290.2006-
dc.identifier.urihttp://hdl.handle.net/10029/6921-
dc.description.abstractEpitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer), previously associated with chlamydial bacterial infection using ultrastructural analysis, was further investigated by using molecular and immunocytochemical methods. Morphologically, all three species showed epitheliocystis cysts in the gills, and barramundi also showed lymphocystis cysts in the skin. From gill cysts of all three species and from skin cysts of barramundi 16S rRNA gene fragments were amplified by PCR and sequenced, which clustered by phylogenetic analysis together with other chlamydia-like organisms in the order Chlamydiales in a lineage separate from the family Chlamydiaceae. By using in situ RNA hybridization, 16S rRNA Chlamydiales-specific sequences were detected in gill cysts of silver perch and in gill and skin cysts of barramundi. By applying immunocytochemistry, chlamydial antigens (lipopolysaccharide and/or membrane protein) were detected in gill cysts of leafy seadragon and in gill and skin cysts of barramundi, but not in gill cysts of silver perch. In conclusion, this is the first time epitheliocystis agents of leafy seadragon, silver perch and barramundi have been undoubtedly identified as belonging to bacteria of the order Chlamydiales by molecular methods. In addition, the results suggested that lymphocystis cysts, known to be caused by iridovirus infection, could be coinfected with the epitheliocystis agent.en
dc.format.extent251508 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleMolecular evidence for association of chlamydiales bacteria with epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer).en
dc.typeArticleen
dc.format.digYES-
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