High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

2.50
Hdl Handle:
http://hdl.handle.net/10029/7094
Title:
High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
Authors:
Aires-de-Sousa, Marta; Boye, K; Lencastre, H de; Deplano, A; Enright, M C; Etienne, J; Friedrich, A; Harmsen, D; Holmes, A; Huijsdens, Xander W; Kearns, A M; Mellmann, A; Meugnier, H; Rasheed, J K; Spalburg, Emile; Strommenger, B; Struelens, M J; Tenover, F C; Thomas, J; Vogel, U; Westh, H; Xu, J; Witte, W
Abstract:
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
Citation:
J. Clin. Microbiol. 2006, 44(2):619-21
Issue Date:
1-Feb-2006
URI:
http://hdl.handle.net/10029/7094
DOI:
10.1128/JCM.44.2.619-621.2006
PubMed ID:
16455927
Type:
Article
Language:
en
ISSN:
0095-1137
Appears in Collections:
Miscellaneous

Full metadata record

DC FieldValue Language
dc.contributor.authorAires-de-Sousa, Marta-
dc.contributor.authorBoye, K-
dc.contributor.authorLencastre, H de-
dc.contributor.authorDeplano, A-
dc.contributor.authorEnright, M C-
dc.contributor.authorEtienne, J-
dc.contributor.authorFriedrich, A-
dc.contributor.authorHarmsen, D-
dc.contributor.authorHolmes, A-
dc.contributor.authorHuijsdens, Xander W-
dc.contributor.authorKearns, A M-
dc.contributor.authorMellmann, A-
dc.contributor.authorMeugnier, H-
dc.contributor.authorRasheed, J K-
dc.contributor.authorSpalburg, Emile-
dc.contributor.authorStrommenger, B-
dc.contributor.authorStruelens, M J-
dc.contributor.authorTenover, F C-
dc.contributor.authorThomas, J-
dc.contributor.authorVogel, U-
dc.contributor.authorWesth, H-
dc.contributor.authorXu, J-
dc.contributor.authorWitte, W-
dc.date.accessioned2007-01-09T15:14:11Z-
dc.date.available2007-01-09T15:14:11Z-
dc.date.issued2006-02-01-
dc.identifier.citationJ. Clin. Microbiol. 2006, 44(2):619-21en
dc.identifier.issn0095-1137-
dc.identifier.pmid16455927-
dc.identifier.doi10.1128/JCM.44.2.619-621.2006-
dc.identifier.urihttp://hdl.handle.net/10029/7094-
dc.description.abstractCurrent DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.en
dc.format.extent86846 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleHigh interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.en
dc.typeArticleen
dc.format.digYES-

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