• Feedback
  • RIVM

Webbased Archive of RIVM Publications

Collection All
Listed communities

Webbased Archive of RIVM Publications > Articles and other publications by RIVM employees > Miscellaneous > High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

Please use this identifier to cite or link to this item: http://hdl.handle.net/10029/7094
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!

Title: High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
Authors: Aires-de-Sousa, Marta
Boye, K
Lencastre, H de
Deplano, A
Enright, M C
Etienne, J
Friedrich, A
Harmsen, D
Holmes, A
Huijsdens, Xander W
Kearns, A M
Mellmann, A
Meugnier, H
Rasheed, J K
Spalburg, Emile
Strommenger, B
Struelens, M J
Tenover, F C
Thomas, J
Vogel, U
Westh, H
Xu, J
Witte, W
Citation: J. Clin. Microbiol. 2006, 44(2):619-21
Issue Date: 1-Feb-2006
URI: http://hdl.handle.net/10029/7094
DOI: 10.1128/JCM.44.2.619-621.2006
PubMed ID: 16455927
Abstract: Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
Type: Article
Language: en
ISSN: 0095-1137
Appears in Collections: Miscellaneous

Files in This Item:
File Description Size Format View/Open
aires.pdf84KbAdobe PDFThumbnail

Related articles on PubMed
Automated DNA sequence-based early warning system for the detection of methicillin-resistant Staphylococcus aureus outbreaks.
Mellmann A, Friedrich AW, Rosenkötter N, Rothgänger J, Karch H, Reintjes R, Harmsen D
2006 Mar
See all 93 articles

All Items in WARP are protected by copyright, with all rights reserved, unless otherwise indicated.