Determination of resorcylic acid lactones in biological samples by GC-MS. Discrimination between illegal use and contamination with fusarium toxins.

2.50
Hdl Handle:
http://hdl.handle.net/10029/7144
Title:
Determination of resorcylic acid lactones in biological samples by GC-MS. Discrimination between illegal use and contamination with fusarium toxins.
Authors:
Blokland, M H; Sterk, S S; Stephany, R W; Launay, F M; Kennedy, D G; Ginkel, L A van
Abstract:
An EU project, FAIR5-CT-1997-3443, has been undertaken to distinguish illegal use of zeranol from consumption of food contaminated with Fusarium spp. toxin. One of the tasks was development of screening and confirmatory methods of analysis. This paper describes a new method based on two-step clean-up and GC-MS analysis. The first clean-up step is matrix-dependant; the second is applicable to both urine and meat. The MS is operated in negative chemical ionisation mode. The method is quantitative for zeranol and taleranol, alpha- and beta-zearalenol, and zearalenone and qualitative for zearalanone. Validation was performed according to the latest EU performance criteria (Commission Decision 2002/657). For analysis of urine CC(alpha) and CC(beta) for the method (microg L(-1)) were 0.06-0.11 for zeranol, 0.07-0.12 for taleranol, 0.07-0.11 for alpha-zearalenol, 0.21-0.36 for beta-zearalenol, 0.35-0.60 for zearalenone, and 0.19-0.33 zearalanone. Within-laboratory reproducibility was 16.2, 11.2, 31.9, 30.1, 26.6, and 54.2% for zeranol, taleranol, alpha-zearalenol, beta-zearalenol, zearalenone, and zearalanone, respectively. It was found that all the compounds are stable in urine at -20 degrees C for at least a year. Part of the validation program was organisation of a small proficiency study (ringtest) and a correlation study with an LC-MS-MS method developed by the Veterinary Science Division (VSD; Belfast, UK-NI). This study showed there was good correlation between results from both laboratories. The method can be used for quantitative analysis discriminating illegal use of zeranol from consumption of zearalenone-contaminated food.
Citation:
Anal Bioanal Chem 2006, 384(5):1221-7
Issue Date:
1-Mar-2006
URI:
http://hdl.handle.net/10029/7144
DOI:
10.1007/s00216-005-0274-4
PubMed ID:
16416095
Type:
Article
Language:
en
ISSN:
1618-2642
Appears in Collections:
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Full metadata record

DC FieldValue Language
dc.contributor.authorBlokland, M H-
dc.contributor.authorSterk, S S-
dc.contributor.authorStephany, R W-
dc.contributor.authorLaunay, F M-
dc.contributor.authorKennedy, D G-
dc.contributor.authorGinkel, L A van-
dc.date.accessioned2007-01-10T09:43:31Z-
dc.date.available2007-01-10T09:43:31Z-
dc.date.issued2006-03-01-
dc.identifier.citationAnal Bioanal Chem 2006, 384(5):1221-7en
dc.identifier.issn1618-2642-
dc.identifier.pmid16416095-
dc.identifier.doi10.1007/s00216-005-0274-4-
dc.identifier.urihttp://hdl.handle.net/10029/7144-
dc.description.abstractAn EU project, FAIR5-CT-1997-3443, has been undertaken to distinguish illegal use of zeranol from consumption of food contaminated with Fusarium spp. toxin. One of the tasks was development of screening and confirmatory methods of analysis. This paper describes a new method based on two-step clean-up and GC-MS analysis. The first clean-up step is matrix-dependant; the second is applicable to both urine and meat. The MS is operated in negative chemical ionisation mode. The method is quantitative for zeranol and taleranol, alpha- and beta-zearalenol, and zearalenone and qualitative for zearalanone. Validation was performed according to the latest EU performance criteria (Commission Decision 2002/657). For analysis of urine CC(alpha) and CC(beta) for the method (microg L(-1)) were 0.06-0.11 for zeranol, 0.07-0.12 for taleranol, 0.07-0.11 for alpha-zearalenol, 0.21-0.36 for beta-zearalenol, 0.35-0.60 for zearalenone, and 0.19-0.33 zearalanone. Within-laboratory reproducibility was 16.2, 11.2, 31.9, 30.1, 26.6, and 54.2% for zeranol, taleranol, alpha-zearalenol, beta-zearalenol, zearalenone, and zearalanone, respectively. It was found that all the compounds are stable in urine at -20 degrees C for at least a year. Part of the validation program was organisation of a small proficiency study (ringtest) and a correlation study with an LC-MS-MS method developed by the Veterinary Science Division (VSD; Belfast, UK-NI). This study showed there was good correlation between results from both laboratories. The method can be used for quantitative analysis discriminating illegal use of zeranol from consumption of zearalenone-contaminated food.en
dc.format.extent203240 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleDetermination of resorcylic acid lactones in biological samples by GC-MS. Discrimination between illegal use and contamination with fusarium toxins.en
dc.typeArticleen
dc.format.digYES-

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