Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period.

2.50
Hdl Handle:
http://hdl.handle.net/10029/7172
Title:
Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period.
Authors:
Horan, Kathleen L; Freeman, Robert; Weigel, Kris; Semret, Makeda; Pfaller, Stacy; Covert, Terry C; Soolingen, Dick van; Leão, Sylvia C; Behr, Marcel A; Cangelosi, Gerard A
Abstract:
The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, these observations also show that strains of the pathogen can be genotypically stable over extended time periods.
Citation:
J. Clin. Microbiol. 2006, 44(3):783-9
Issue Date:
1-Mar-2006
URI:
http://hdl.handle.net/10029/7172
DOI:
10.1128/JCM.44.3.783-789.2006
PubMed ID:
16517855
Type:
Article
Language:
en
ISSN:
0095-1137
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorHoran, Kathleen L-
dc.contributor.authorFreeman, Robert-
dc.contributor.authorWeigel, Kris-
dc.contributor.authorSemret, Makeda-
dc.contributor.authorPfaller, Stacy-
dc.contributor.authorCovert, Terry C-
dc.contributor.authorSoolingen, Dick van-
dc.contributor.authorLeão, Sylvia C-
dc.contributor.authorBehr, Marcel A-
dc.contributor.authorCangelosi, Gerard A-
dc.date.accessioned2007-01-10T11:59:38Z-
dc.date.available2007-01-10T11:59:38Z-
dc.date.issued2006-03-01-
dc.identifier.citationJ. Clin. Microbiol. 2006, 44(3):783-9en
dc.identifier.issn0095-1137-
dc.identifier.pmid16517855-
dc.identifier.doi10.1128/JCM.44.3.783-789.2006-
dc.identifier.urihttp://hdl.handle.net/10029/7172-
dc.description.abstractThe genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, these observations also show that strains of the pathogen can be genotypically stable over extended time periods.en
dc.format.extent603710 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleIsolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period.en
dc.typeArticleen
dc.format.digYES-
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