Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms.

2.50
Hdl Handle:
http://hdl.handle.net/10029/7175
Title:
Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms.
Authors:
Semret, Makeda; Turenne, Christine Y; Haas, Petra de; Collins, Desmond M; Behr, Marcel A
Abstract:
The Mycobacterium avium species consists of a group of organisms that are genetically related but phenotypically diverse, with certain variants presenting clear differences in terms of their host association and disease manifestations. The ability to distinguish between these subtypes is of relevance for accurate diagnosis and for control programs. Using a comparative genomics approach, we have uncovered large sequence polymorphisms that are, respectively, absent from bird-type M. avium isolates and from cattle types and sheep types of M. avium subsp. paratuberculosis. By evaluating the distribution of these genomic polymorphisms across a panel of strains, we were able to assign unique genomic signatures to these host-associated variants. We propose a simple PCR-based strategy based on these polymorphisms that can rapidly type M. avium isolates into these subgroups.
Citation:
J. Clin. Microbiol. 2006, 44(3):881-7
Issue Date:
1-Mar-2006
URI:
http://hdl.handle.net/10029/7175
DOI:
10.1128/JCM.44.3.881-887.2006
PubMed ID:
16517871
Type:
Article
Language:
en
ISSN:
0095-1137
Appears in Collections:
Infectious Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorSemret, Makeda-
dc.contributor.authorTurenne, Christine Y-
dc.contributor.authorHaas, Petra de-
dc.contributor.authorCollins, Desmond M-
dc.contributor.authorBehr, Marcel A-
dc.date.accessioned2007-01-10T12:24:40Z-
dc.date.available2007-01-10T12:24:40Z-
dc.date.issued2006-03-01-
dc.identifier.citationJ. Clin. Microbiol. 2006, 44(3):881-7en
dc.identifier.issn0095-1137-
dc.identifier.pmid16517871-
dc.identifier.doi10.1128/JCM.44.3.881-887.2006-
dc.identifier.urihttp://hdl.handle.net/10029/7175-
dc.description.abstractThe Mycobacterium avium species consists of a group of organisms that are genetically related but phenotypically diverse, with certain variants presenting clear differences in terms of their host association and disease manifestations. The ability to distinguish between these subtypes is of relevance for accurate diagnosis and for control programs. Using a comparative genomics approach, we have uncovered large sequence polymorphisms that are, respectively, absent from bird-type M. avium isolates and from cattle types and sheep types of M. avium subsp. paratuberculosis. By evaluating the distribution of these genomic polymorphisms across a panel of strains, we were able to assign unique genomic signatures to these host-associated variants. We propose a simple PCR-based strategy based on these polymorphisms that can rapidly type M. avium isolates into these subgroups.en
dc.format.extent459251 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleDifferentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms.en
dc.typeArticleen
dc.format.digYES-

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