Response of spontaneously hypertensive rats to inhalation of fine and ultrafine particles from traffic: experimental controlled study.

2.50
Hdl Handle:
http://hdl.handle.net/10029/7616
Title:
Response of spontaneously hypertensive rats to inhalation of fine and ultrafine particles from traffic: experimental controlled study.
Authors:
Kooter, Ingeborg M; Boere, A John F; Fokkens, Paul Hb; Leseman, Daan Lac; Dormans, Jan Ama; Cassee, Flemming R
Abstract:
ABSTRACT: BACKGROUND: Many epidemiological studies have shown that mass concentrations of ambient particulate matter (PM) are associated with adverse health effects in the human population. Since PM is still a very crude measure, this experimental study has explored the role of two distinct size fractions: ultrafine (<0.15 mum) and fine (0.15- 2.5 mum) PM. In a series of 2-day inhalation studies, spontaneously hypersensitive (SH) rats were exposed to fine, concentrated, ambient PM (fCAP) at a city background location or a combination of ultrafine and fine (u+fCAP) PM at a location dominated by traffic. We examined the effect on inflammation and both pathological and haematological indicators as markers of pulmonary and cardiovascular injury. Exposure concentrations ranged from 399 mug/m3 to 3613 mug/m3 for fCAP and from 269mug/m3 to 556 mug/m3 for u+fCAP. RESULTS: Ammonium, nitrate, and sulphate ions accounted for 56 +/- 16% of the total fCAP mass concentrations, but only 17 +/- 6% of the u+fCAP mass concentrations. Unambiguous particle uptake in alveolar macrophages was only seen after u+fCAP exposures. Neither fCAP nor u+fCAP induced significant changes of cytotoxicity or inflammation in the lung. However, markers of oxidative stress (heme oxygenase-1 and malondialdehyde) were affected by both fCAP and u+fCAP exposure, although not always significantly. Additional analysis revealed heme oxygenase-1 (HO-1) levels that followed a nonmonotonic function with an optimum at around 600 mug/m3 for fCAP. As a systemic response, exposure to u+fCAP and fCAP resulted in significant decreases of the white blood cell concentrations. CONCLUSION: Minor pulmonary and systemic effects are observed after both fine and ultrafine + fine PM exposure. These effects do not linearly correlate with the CAP mass. A greater component of traffic CAP and/or a larger proportion ultrafine PM does not strengthen the absolute effects.
Citation:
Part Fibre Toxicol 2006, 3:7
Issue Date:
2006
URI:
http://hdl.handle.net/10029/7616
DOI:
10.1186/1743-8977-3-7
PubMed ID:
16700918
Type:
Article
Language:
en
ISSN:
1743-8977
Appears in Collections:
Environment

Full metadata record

DC FieldValue Language
dc.contributor.authorKooter, Ingeborg M-
dc.contributor.authorBoere, A John F-
dc.contributor.authorFokkens, Paul Hb-
dc.contributor.authorLeseman, Daan Lac-
dc.contributor.authorDormans, Jan Ama-
dc.contributor.authorCassee, Flemming R-
dc.date.accessioned2007-01-18T12:18:51Z-
dc.date.available2007-01-18T12:18:51Z-
dc.date.issued2006-
dc.identifier.citationPart Fibre Toxicol 2006, 3:7en
dc.identifier.issn1743-8977-
dc.identifier.pmid16700918-
dc.identifier.doi10.1186/1743-8977-3-7-
dc.identifier.urihttp://hdl.handle.net/10029/7616-
dc.description.abstractABSTRACT: BACKGROUND: Many epidemiological studies have shown that mass concentrations of ambient particulate matter (PM) are associated with adverse health effects in the human population. Since PM is still a very crude measure, this experimental study has explored the role of two distinct size fractions: ultrafine (<0.15 mum) and fine (0.15- 2.5 mum) PM. In a series of 2-day inhalation studies, spontaneously hypersensitive (SH) rats were exposed to fine, concentrated, ambient PM (fCAP) at a city background location or a combination of ultrafine and fine (u+fCAP) PM at a location dominated by traffic. We examined the effect on inflammation and both pathological and haematological indicators as markers of pulmonary and cardiovascular injury. Exposure concentrations ranged from 399 mug/m3 to 3613 mug/m3 for fCAP and from 269mug/m3 to 556 mug/m3 for u+fCAP. RESULTS: Ammonium, nitrate, and sulphate ions accounted for 56 +/- 16% of the total fCAP mass concentrations, but only 17 +/- 6% of the u+fCAP mass concentrations. Unambiguous particle uptake in alveolar macrophages was only seen after u+fCAP exposures. Neither fCAP nor u+fCAP induced significant changes of cytotoxicity or inflammation in the lung. However, markers of oxidative stress (heme oxygenase-1 and malondialdehyde) were affected by both fCAP and u+fCAP exposure, although not always significantly. Additional analysis revealed heme oxygenase-1 (HO-1) levels that followed a nonmonotonic function with an optimum at around 600 mug/m3 for fCAP. As a systemic response, exposure to u+fCAP and fCAP resulted in significant decreases of the white blood cell concentrations. CONCLUSION: Minor pulmonary and systemic effects are observed after both fine and ultrafine + fine PM exposure. These effects do not linearly correlate with the CAP mass. A greater component of traffic CAP and/or a larger proportion ultrafine PM does not strengthen the absolute effects.en
dc.format.extent276087 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleResponse of spontaneously hypertensive rats to inhalation of fine and ultrafine particles from traffic: experimental controlled study.en
dc.typeArticleen
dc.format.digYES-

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