Evaluatie van de Quantase R Phenylalanine bepaling en het Millipore Multiscreen Assay System als methode voor de screening van pasgeborenen op PKU

2.50
Hdl Handle:
http://hdl.handle.net/10029/9900
Title:
Evaluatie van de Quantase R Phenylalanine bepaling en het Millipore Multiscreen Assay System als methode voor de screening van pasgeborenen op PKU
Authors:
Elvers LH; Diependaal GAM; Blonk HJ; Loeber JG
Other Titles:
[Evaluation of the Quantase R Phenylalanine assay and the Millipore Multiscreen Assay System for the neonatal screening on PKU.]
Abstract:
The Quantase R Phenylalanine Assay, an enzymatic/colorimetric method for the determination of phenylalanine (Phe) in dried bloodspots, was evaluated for the neonatal screening on PKU. The within run variation (N=12) for bloodspot samples with 0.15, 0.29 and 0.53 mmol/l was 10%, 5% and 5%, respectively. The between run variation (N=56) for bloodspot samples with 0.38 and 0.96 mmol/l was 9% and 8%, respectively. The mean concentration and standarddeviation in 33630 bloodspot samples was 0.077 +- 0.025 mmol/l (%CV=32%). The measured Phe-concentration was elevated (>=0.24 mmol/l) in 13 samples (0.039%). Reanalysis of these samples, in the same bloodspots, revealed a Phe-concentration of <0.12 mmol/l in 6 samples. These 6 samples were also found to be 'negative' in the Guthrie-test. Therefore, 6 samples (0.018%) were 'false-elevated'. For 5 of these samples no explanation was found. In 5770 bloodspot samples Guthrie test. All 'negative' samples in the Quantase were also 'negative' in the Guthrie. According to the manufacturer the absorbance has to be read at 570 nm. To reduce the number of samples with a 'false-elevated' concentration, we recommend to read the absorbances both at 570 nm and at 690 nm to correct for non-specific absorbance (dual wavelength). We used the Millipore Multiscreen Assay System for the extraction of the phenylalanine from the bloodspots. This system appeared to function well in practive. The special filterplates are rather expensive, but under stringent conditions the plates can be recycled three times. A visual control on the correct transfer of the extracts is necessary.
Affiliation:
TEP
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
31-Jan-1994
URI:
http://hdl.handle.net/10029/9900
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/199003029.html
Language:
nl
Series/Report no.:
RIVM Rapport 199003029
Appears in Collections:
RIVM reports - old archive

Full metadata record

DC FieldValue Language
dc.contributor.authorElvers LHen_US
dc.contributor.authorDiependaal GAMen_US
dc.contributor.authorBlonk HJen_US
dc.contributor.authorLoeber JGen_US
dc.date.accessioned2007-03-09T15:36:24Z-
dc.date.available2007-03-09T15:36:24Z-
dc.date.issued1994-01-31en_US
dc.identifier199003029en_US
dc.identifier.urihttp://hdl.handle.net/10029/9900-
dc.description.abstractThe Quantase R Phenylalanine Assay, an enzymatic/colorimetric method for the determination of phenylalanine (Phe) in dried bloodspots, was evaluated for the neonatal screening on PKU. The within run variation (N=12) for bloodspot samples with 0.15, 0.29 and 0.53 mmol/l was 10%, 5% and 5%, respectively. The between run variation (N=56) for bloodspot samples with 0.38 and 0.96 mmol/l was 9% and 8%, respectively. The mean concentration and standarddeviation in 33630 bloodspot samples was 0.077 +- 0.025 mmol/l (%CV=32%). The measured Phe-concentration was elevated (>=0.24 mmol/l) in 13 samples (0.039%). Reanalysis of these samples, in the same bloodspots, revealed a Phe-concentration of <0.12 mmol/l in 6 samples. These 6 samples were also found to be 'negative' in the Guthrie-test. Therefore, 6 samples (0.018%) were 'false-elevated'. For 5 of these samples no explanation was found. In 5770 bloodspot samples Guthrie test. All 'negative' samples in the Quantase were also 'negative' in the Guthrie. According to the manufacturer the absorbance has to be read at 570 nm. To reduce the number of samples with a 'false-elevated' concentration, we recommend to read the absorbances both at 570 nm and at 690 nm to correct for non-specific absorbance (dual wavelength). We used the Millipore Multiscreen Assay System for the extraction of the phenylalanine from the bloodspots. This system appeared to function well in practive. The special filterplates are rather expensive, but under stringent conditions the plates can be recycled three times. A visual control on the correct transfer of the extracts is necessary.en
dc.format.extent1356175 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isonlen_US
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVMen_US
dc.relation.ispartofseriesRIVM Rapport 199003029en_US
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/199003029.htmlen_US
dc.subject.otherphenylketonuriaen
dc.subject.otherneonatal screeningen
dc.subject.otherassayen
dc.subject.otherevaluation studiesen
dc.subject.otherquantase r phenylalanine assayen
dc.subject.othermillipore multiscreen assay systemen
dc.subject.otherhielpriken
dc.subject.otherphenylketonurienl
dc.subject.otherneonatale screeningnl
dc.subject.otherbepalingnl
dc.subject.otherevaluatienl
dc.titleEvaluatie van de Quantase R Phenylalanine bepaling en het Millipore Multiscreen Assay System als methode voor de screening van pasgeborenen op PKUen_US
dc.title.alternative[Evaluation of the Quantase R Phenylalanine assay and the Millipore Multiscreen Assay System for the neonatal screening on PKU.]en_US
dc.contributor.departmentTEPen_US
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