Optimalisering van de meticillinegevoeligheidsbepaling bij coaguase negatieve staphylococcen

2.50
Hdl Handle:
http://hdl.handle.net/10029/9939
Title:
Optimalisering van de meticillinegevoeligheidsbepaling bij coaguase negatieve staphylococcen
Authors:
Veen-Rutgers A van; Sijbolts FH; Schot CS; Schouls LM; Neeling AJ de; Klingeren B van
Other Titles:
[Optimization of the methicillin sensitivity determination of coaguase negative staphylococci.]
Abstract:
Coagulase negative staphylococci are increasingly found to be involved in nosocomial infections, especially after invasive surgery. Since the prevalence of methicillin resistance among these strains is high, there is a need for a fast and reliable method to detect this resistance. In this investigation 40 CNS strains, consisting of S.epidermidis and S.haemolyticus, were tested using the E-test and the agardilution test. The tests were carried out with methicillin and oxacillin on Mueller Hinton agar, Columbia and PDM agar supplied with 0, 2 and 5% sodium chloride, inocula of 106 en 108 cfu/ml and MIC reading after 24 and 48 h incubation. The presence of the mecA gene, the structural gene for PBP2a, responsible for methicillin resistance in staphylococcus species, was determined by a PCR method and served as reference (golden standard) for methicillin resistance of the isolates. The most reliable results to detect methicillin resistance in these coagulase negative staphylococcus strains were obtained with the E-test as well as the agar dilution test on Mueller Hinton agar supplemented with 2% NaCl, an inoculum of 108 cfu/ml, an incubation temperature of 37 degrees C and an incubation period of 24 h or 48 h (if sensitive after 24 h). The test was more reliable with oxacillin than with methicillin, particularly in the E-test.
Affiliation:
LBA; MMB; Diaconessenhuis Utrecht
Publisher:
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Issue Date:
30-Jun-1994
URI:
http://hdl.handle.net/10029/9939
Additional Links:
http://www.rivm.nl/bibliotheek/rapporten/253601002.html
Language:
nl
Series/Report no.:
RIVM Rapport 253601002
Appears in Collections:
RIVM reports - old archive

Full metadata record

DC FieldValue Language
dc.contributor.authorVeen-Rutgers A vanen_US
dc.contributor.authorSijbolts FHen_US
dc.contributor.authorSchot CSen_US
dc.contributor.authorSchouls LMen_US
dc.contributor.authorNeeling AJ deen_US
dc.contributor.authorKlingeren B vanen_US
dc.date.accessioned2007-03-09T15:42:48Z-
dc.date.available2007-03-09T15:42:48Z-
dc.date.issued1994-06-30en_US
dc.identifier253601002en_US
dc.identifier.urihttp://hdl.handle.net/10029/9939-
dc.description.abstractCoagulase negative staphylococci are increasingly found to be involved in nosocomial infections, especially after invasive surgery. Since the prevalence of methicillin resistance among these strains is high, there is a need for a fast and reliable method to detect this resistance. In this investigation 40 CNS strains, consisting of S.epidermidis and S.haemolyticus, were tested using the E-test and the agardilution test. The tests were carried out with methicillin and oxacillin on Mueller Hinton agar, Columbia and PDM agar supplied with 0, 2 and 5% sodium chloride, inocula of 106 en 108 cfu/ml and MIC reading after 24 and 48 h incubation. The presence of the mecA gene, the structural gene for PBP2a, responsible for methicillin resistance in staphylococcus species, was determined by a PCR method and served as reference (golden standard) for methicillin resistance of the isolates. The most reliable results to detect methicillin resistance in these coagulase negative staphylococcus strains were obtained with the E-test as well as the agar dilution test on Mueller Hinton agar supplemented with 2% NaCl, an inoculum of 108 cfu/ml, an incubation temperature of 37 degrees C and an incubation period of 24 h or 48 h (if sensitive after 24 h). The test was more reliable with oxacillin than with methicillin, particularly in the E-test.en
dc.format.extent1526000 bytesen_US
dc.format.extent1562248 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isonlen_US
dc.publisherRijksinstituut voor Volksgezondheid en Milieu RIVMen_US
dc.relation.ispartofseriesRIVM Rapport 253601002en_US
dc.relation.urlhttp://www.rivm.nl/bibliotheek/rapporten/253601002.htmlen_US
dc.subject.otherstaphylococcus epidermisen
dc.subject.othermicrobial drug resistanceen
dc.subject.othermethicillinen
dc.subject.otheroxacillinen
dc.subject.othermethicillin resistanceen
dc.subject.otherstaphylococcus epidermisnl
dc.subject.otherresistentienl
dc.subject.othermeticillinenl
dc.subject.otheroxacillinenl
dc.titleOptimalisering van de meticillinegevoeligheidsbepaling bij coaguase negatieve staphylococcenen_US
dc.title.alternative[Optimization of the methicillin sensitivity determination of coaguase negative staphylococci.]en_US
dc.contributor.departmentLBAen_US
dc.contributor.departmentMMBen_US
dc.contributor.departmentDiaconessenhuis Utrechten_US
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