The assessment for potential thyroid-mediated endocrine disruption in amphibians: Clarification on the use of new methods and on the interpretation of changes in thyroid histology
Baumann, Lisa Baynes, Alice Bennekou, Susanne Hougaard Crofton, Kevin Dang, Zhichao Degitz, Sigmund Fini, Jean-Baptiste Gilbert, Mary Holbech, Henrik Terron, Andrea
Baumann, Lisa
Baynes, Alice
Bennekou, Susanne Hougaard
Crofton, Kevin
Dang, Zhichao
Degitz, Sigmund
Fini, Jean-Baptiste
Gilbert, Mary
Holbech, Henrik
Terron, Andrea
Series / Report no.
Open Access
Type
Journal Article
Article
Article
Language
en
Date of publication
2025-12-11
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
The assessment for potential thyroid-mediated endocrine disruption in amphibians: Clarification on the use of new methods and on the interpretation of changes in thyroid histology
Translated Title
Published in
EFSA J 2025; 23(12):e9815
Abstract
Amphibians (specifically ) are used as the model species to assess potential endocrine-disrupting properties in non-mammalian species through thyroid modality. The amphibian metamorphosis assay is the most frequently available test. Attempts have been made to modify this protocol in order to make it more fit for purpose and overcome potential limitations. In light of these developments, EFSA, with the support of the Working Group on Endocrine Disruptors, under the auspices of a self-task mandate here endeavours to clarify the pros and cons of newly proposed amphibian protocols when compared with the standard guideline tests. Moreover, recommendations to facilitate the interpretation of findings in relation to changes in thyroid histopathology have been included.