Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study
van Veen, Anneloes; Shahab, Selvi N; Rijfkogel, Amber; Voor In 't Holt, Anne F; Klaassen, Corné HW; Vos, Margreet C; Saharman, Yulia Rosa; Karuniawati, Anis; Zelli, Silvia; De Lorenzis, Desy; Menchinelli, Giulia; De Angelis, Giulia; Sanguinetti, Maurizio; Kemper, Merel; de Jong, Anniek EE; Mohammadi, Sima; Renaud, Valentine; Kukavica-Ibrulj, Irena; Potvin, Marianne; Nguyen, Guillaume Q; Gauthier, Jeff; Levesque, Roger C; Schmitt, Heike; Severin, Juliëtte A
Series / Report no.
Open Access
Type
Journal Article
Article
Article
Language
en
Date
2025-01-15
Research Projects
Organizational Units
Journal Issue
Title
Sources and Transmission Routes of Carbapenem-Resistant Pseudomonas aeruginosa: Study Design and Methodology of the SAMPAN Study
Translated Title
Published in
Antibiotics 2025; 14(1):94
Abstract
: The global spread of carbapenem-resistant (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study ("A Smart Surveillance Strategy for Carbapenem-resistant ") is to develop a globally applicable surveillance strategy. : First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals' (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. : The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.