Highly sensitive parechovirus CODEHOP PCR amplification of the complete VP1 gene for typing directly from clinical specimens and correct typing based on phylogenetic clustering.
Cremer, Jeroen Morley, Ursula Pas, Suzan Wolthers, Katja Vennema, Harry Duizer, Erwin Benschop, Kimberley
Cremer, Jeroen
Morley, Ursula
Pas, Suzan
Wolthers, Katja
Vennema, Harry
Duizer, Erwin
Benschop, Kimberley
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Open Access
Type
Article
Language
en
Date of publication
2019-08-01
Year of publication
Research Projects
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Journal Issue
Title
Highly sensitive parechovirus CODEHOP PCR amplification of the complete VP1 gene for typing directly from clinical specimens and correct typing based on phylogenetic clustering.
Translated Title
Published in
J Med Microbiol 2019; 68(8):1194-203
Abstract
The assay was HPeV-specific and has a sensitivity of 6.3 TCID50 ml-1 for HPeV1 and 0.63 TCID50 ml-1 for HPeV3. Analysis of the complete VP1 gene in comparison to partial VP1 fragments generated by previously published PCRs showed homologous clustering for most types. However, phylogenetic analysis of partial VP1 fragments showed incongruent typing based on the 75 % homology classification rule. In particular, the strains designated as type 17 were found to be either type 3 or 4 when using the (near-) complete VP1 fragment.