Geautomatiseerde monsterzuivering door kolomschakeling bij de bepaling van lorazepam in ratteserum met hoge-prestatie vloeistofchromatografie
Citations
Altmetric:
Series / Report no.
Open Access
Type
Report
Language
nl
Date
1990-01-31
Research Projects
Organizational Units
Journal Issue
Title
Geautomatiseerde monsterzuivering door
kolomschakeling bij de bepaling van lorazepam in ratteserum met
hoge-prestatie vloeistofchromatografie
Translated Title
Automated sample clean-up by column switching in
the determination of lorazepam in rat serum with high-performance liquid
chromatography
Published in
Abstract
Abstract niet beschikbaar
In this report a study is described on the application of automated sample clean up by column switching in the quantitative determination of lorazepam in rat serum. Extraction of lorazepam from serum is by using a solid-phase extraction procedure with an "High-Hydrophobic" octadecylsilane sorbent. Determination of lorazepam is with high- performance liquid chromatography. The solid-phase extract is separated over a precolumn from serum components by "on line" column switching. Detection is by UV-detection at 230 nm. The extraction recovery (+- standard deviation) is (91 +- 0.7)%. The precision is 2.4% at 1.12 mug/l. The method is adapted to a concentration range from 0.05 to 4.2 mug/l.
In this report a study is described on the application of automated sample clean up by column switching in the quantitative determination of lorazepam in rat serum. Extraction of lorazepam from serum is by using a solid-phase extraction procedure with an "High-Hydrophobic" octadecylsilane sorbent. Determination of lorazepam is with high- performance liquid chromatography. The solid-phase extract is separated over a precolumn from serum components by "on line" column switching. Detection is by UV-detection at 230 nm. The extraction recovery (+- standard deviation) is (91 +- 0.7)%. The precision is 2.4% at 1.12 mug/l. The method is adapted to a concentration range from 0.05 to 4.2 mug/l.
Description
Publisher
Sponsors
HIG
CBG