De invloed van zaagselbodem- of draadbodemkooien op de kinetiek van benzo(a)pyreen bij de rat
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Series / Report no.
Open Access
Type
Report
Language
nl
Date
1996-01-31
Research Projects
Organizational Units
Journal Issue
Title
De invloed van zaagselbodem- of draadbodemkooien op
de kinetiek van benzo(a)pyreen bij de rat
Translated Title
The influence of the use of animal cages with wire
floor or macrolon cages with middle size saw on the kinetics of
benzo(a)pyrene in the rat
Published in
Abstract
Ter ondersteuning van een chronisch
carcinogeniteitsonderzoek bij de rat, werd een onderzoek verricht naar de
invloed van het gebruik van draadbodemkooien of macrolonbakken met
middelgrof zaagsel op de kinetiek van benzo(a)pyreen in de rat na orale
toediening. Bij de experimenten werden 12 ratten, 6 mannetjes en 6
vrouwtjes, gebruikt. De ratten werden onderworpen aan drie behandelingen:
verblijf in draadbodemkooi en intraveneuze benzo(a)pyreen toediening
(behandeling A), verblijf in draadbodemkooien en orale toediening van
benzo(a)pyreen (behandeling B) en verblijf in macrolonkooien met middelgrof
zaagsel bodembedekking en orale toediening (behandeling C). Het experiment
werd als een drieweg gekruiste gerandomiseerde proef opgezet met
uitwasperioden van een week. Aan het einde van elke behandelingsperiode
werd bloed afgenomen. Na centrifugatie van het bloed werd het verkregen
plasma vervolgens bij -20 C opgeslagen totdat de monsters met behulp HPLC
werden geanalyseerd. Op grond van de verkregen benzo(a)pyreen concentraties
werd een aantal farmacokinetische kengetallen (AUC 0-t C-max t-max F en MRT)
berekend. Het blijkt dat er alleen bij de C-max een statistisch significant
verschil optreedt tussen behandeling B en C. Tussen mannelijke en
vrouwelijke ratten bleken voor de genoemde farmacokinetische kentallen geen
significante verschillen op te treden. Er kan tenslotte geconcludeerd
worden dat het voor de biobeschikbaarheid niet uitmaakt of de ratten op
draadbodem- of op zaagselbodemkooien met middelgrof zaagsel
verblijven.
To support a chronical carcinogenicity experiment in the rat, an investigation into the influence of the use of either animal cages with wire floor or macrolon cages with middle size saw on the kinetics of oral benzo(a)pyrene in the rat was carried out. Twelve rats, six males and six females, were used in the experiments. The rats were subjected to three different treatments: housing in animal cages with wire floor and intravenous administration of benzo(a)pyrene (treatment A), housing in cages with wire floor and oral administration of benzo(a)pyrene (treatment B) and housing in macrolon cages with middle size saw and oral administration (treatment C). The study was designed as a three way, randomised cross-over experiment with wash-out periods of one week between the treatments. At the end of each period of treatment blood samples were taken. After centrifugation the plasma was stored at -20 degrees C until the samples were analysed by HPLC. On the basis of the concentrations of benzo(a)pyrene obtained a number of pharmacokinetic data (AUC 0-t, C-max, t-max, F and MRT) was calculated. Only at the C-max there was a statistically significant difference between treatment B and C. No significant differences appeared to be present between male and female rats. Finally, the conclusion is that there is no influence on bioavailability if rats are housed in macrolon cages with middle size saw as compared to wire floor.
To support a chronical carcinogenicity experiment in the rat, an investigation into the influence of the use of either animal cages with wire floor or macrolon cages with middle size saw on the kinetics of oral benzo(a)pyrene in the rat was carried out. Twelve rats, six males and six females, were used in the experiments. The rats were subjected to three different treatments: housing in animal cages with wire floor and intravenous administration of benzo(a)pyrene (treatment A), housing in cages with wire floor and oral administration of benzo(a)pyrene (treatment B) and housing in macrolon cages with middle size saw and oral administration (treatment C). The study was designed as a three way, randomised cross-over experiment with wash-out periods of one week between the treatments. At the end of each period of treatment blood samples were taken. After centrifugation the plasma was stored at -20 degrees C until the samples were analysed by HPLC. On the basis of the concentrations of benzo(a)pyrene obtained a number of pharmacokinetic data (AUC 0-t, C-max, t-max, F and MRT) was calculated. Only at the C-max there was a statistically significant difference between treatment B and C. No significant differences appeared to be present between male and female rats. Finally, the conclusion is that there is no influence on bioavailability if rats are housed in macrolon cages with middle size saw as compared to wire floor.
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