Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds
Volfová, Karolina Hönig, Václav Houda, Michal Papežík, Petr Lesiczka, Paulina Maria Fonville, Manoj Sprong, Hein Bolfíková, Barbora Černá Hulva, Pavel Růžek, Daniel
Volfová, Karolina
Hönig, Václav
Houda, Michal
Papežík, Petr
Lesiczka, Paulina Maria
Fonville, Manoj
Sprong, Hein
Bolfíková, Barbora Černá
Hulva, Pavel
Růžek, Daniel
Series / Report no.
Open Access
Type
Article
Language
en
Date
2026-01-12
Research Projects
Organizational Units
Journal Issue
Title
Turning urban wildlife mortality into a surveillance tool: Detection of vector-borne pathogens in carcasses of hedgehogs, squirrels, and blackbirds
Translated Title
Published in
One Health 2026; 22:101328
Abstract
Tick-borne zoonoses pose a major challenge to human and animal health, driving efforts to monitor the distribution, intensity, and diversity of their causative agents. Within the One Health framework, which links human, animal, and environmental health, integrated surveillance strategies are increasingly needed. However, most studies focus on tick vectors, while vertebrate reservoirs are often overlooked due to labour-intensive sampling, the need for specialized skills, and legislative or species protection constraints.
This study evaluated whether carcasses of accidentally killed wildlife (primarily roadkill) can serve as a source of biological material for vector-borne pathogen surveillance, with a focus on urban habitats due to their public health relevance. Hedgehogs, squirrels, and blackbirds were selected as synanthropic species that thrive in cities, are commonly infested by ticks, and act as hosts for zoonotic tick-borne pathogens (TBPs).
A total of 268 carcasses (125 hedgehogs, 55 squirrels, and 88 blackbirds) were collected across multiple Czech cities with public assistance. Overall, 1836 tissue samples were analyzed using multiplex real-time PCR assays targeting over ten microorganisms. Detection efficiency was compared across tissues, with ear and skin consistently the most reliable and versatile sample types. Individual pathogen-host-tissue combinations reached 65–93% efficiency, highlighting the value of multi-tissue sampling. The most prevalent TBPs detected were Anaplasma phagocytophilum, Borrelia burgdorferi s.l., Bartonella spp., and Rickettsia helvetica.
In conclusion, carcasses of accidentally killed urban wildlife provide a practical and valuable resource for TBP surveillance, complementing vector-focused methods. This approach supports One Health principles by integrating wildlife monitoring into urban disease surveillance efforts.