Cooperative Study 17beta-Estradiol in Bovine Serum
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Series / Report no.
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Type
Report
Language
en
Date
1997-08-31
Research Projects
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Journal Issue
Title
Cooperative Study 17beta-Estradiol in Bovine
Serum
Translated Title
Cooperatief onderzoek 17beta-estradiol in
runderserum
Published in
Abstract
Dit rapport beschrijft de resultaten van een in 1995
georganiseerde cooperatieve studie '17beta-estradiol in runderserum'. Het
doel van deze ringtest was het evalueren van de huidige analytische
mogelijkheden van verantwoordelijke controlerende laboratoria, zowel
Routine- of Veldlaboratoria (RFL) als Nationale Referentie Laboratoria (NRL)
met betrekking tot de controle op aanwezigheid van residuen van
17beta-estradiol. Voor dit doel werden vijf gevriesdroogde monsters serum,
met verschillende massa concentraties 17beta-estradiol, bereid en
gedistribueerd over 19 deelnemende laboratoria. Twee verschillende
technieken werden gebruikt: (radio) immunochemische methoden en methoden
gebaseerd op gas chromatografie-massaspectrometrie (GC-MS). De laboratoria
die werkten met een GC-MS-methode vonden massa concentraties met een Z-score
tussen -2 and +2 (Z < 2). Dit betekent dat er goede overeenstemming was
tussen de laboratoria. De meeste laboratoria die een (R)IA-methode
toepasten vonden concentraties met een Z-score tussen -2 and +2 (goede
overeenstemming onderling). Een klein aantal laboratoria rapporteerden een
afwijkend resultaat (2 < Z < 3).
This report describes the results of a cooperative study organised in 1995. The objective of this study was to evaluate the analytical possibilities within the responsible control laboratories (Routine or Field Laboratories (RFLs) and National Reference Laboratories (NRLs) with respect to the control for residues of 17beta-estradiol. For this purpose five different samples of lyophilised bovine serum, containing different mass concentrations of 17beta-estradiol, were prepared and distributed among 19 participating laboratories. The laboratories were asked to analyse the samples with their in-house procedures. The evaluation is based on Z-scores, a quantitative value describing the difference between an individual value from the overall average value, in relation to the standard deviation of the population of results. As for GC-MS all laboratories found mass concentrations with a Z-score between -2 and +2 (Z < 2) indicating good agreement between laboratories. As for (R)IA most laboratories found mass concentrations with a Z-score between -2 and +2 indicating good agreement. A limited number of laboratories reported a doubtful result (2 < Z < 3).
This report describes the results of a cooperative study organised in 1995. The objective of this study was to evaluate the analytical possibilities within the responsible control laboratories (Routine or Field Laboratories (RFLs) and National Reference Laboratories (NRLs) with respect to the control for residues of 17beta-estradiol. For this purpose five different samples of lyophilised bovine serum, containing different mass concentrations of 17beta-estradiol, were prepared and distributed among 19 participating laboratories. The laboratories were asked to analyse the samples with their in-house procedures. The evaluation is based on Z-scores, a quantitative value describing the difference between an individual value from the overall average value, in relation to the standard deviation of the population of results. As for GC-MS all laboratories found mass concentrations with a Z-score between -2 and +2 (Z < 2) indicating good agreement between laboratories. As for (R)IA most laboratories found mass concentrations with a Z-score between -2 and +2 indicating good agreement. A limited number of laboratories reported a doubtful result (2 < Z < 3).
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