Impaired JAK-STAT pathway signaling in leukocytes of the frail elderly.
Samson, Leonard Daniël Engelfriet, Peter Verschuren, W M Monique Picavet, H Susan J Ferreira, José A de Zeeuw-Brouwer, Mary-Lène Buisman, Anne-Marie Boots, A Mieke H
Samson, Leonard Daniël
Engelfriet, Peter
Verschuren, W M Monique
Picavet, H Susan J
Ferreira, José A
de Zeeuw-Brouwer, Mary-Lène
Buisman, Anne-Marie
Boots, A Mieke H
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Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2022-01-17
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
Impaired JAK-STAT pathway signaling in leukocytes of the frail elderly.
Translated Title
Published in
Immunity & ageing : I & A
Abstract
Elderly often show reduced immune functioning and can develop chronic low-grade inflammation. Why some elderly are more prone to become frail is unknown. We investigated whether frailty is associated with altered cytokine signaling through the JAK-STAT pathway in leukocytes of 34 individuals aged 65-74 years. In addition, we investigated how this relation is affected by chronic low-grade inflammation during the previous 20 years. Cytokine signaling was quantified by measuring intracellular STAT1, STAT3, and STAT5 phosphorylation in monocytes, B cells, CD4+ T cells and CD8+ T cells upon stimulation with IL-2, IL-6, IL-10, IFNα and IFNγ, using phospho-flow cytometry. Presence of chronic low-grade inflammation was investigated by evaluating 18 different plasma inflammatory markers that had been measured repeatedly in the same individuals over the previous 20 years. Frailty was assessed as a score on a frailty index.