PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotype.
Wagner, Arnika K Kadri, Nadir Tibbitt, Chris van de Ven, Koen Bagawath-Singh, Sunitha Oliinyk, Denys LeGresley, Eric Campbell, Nicole Trittel, Stephanie Riese, Peggy
Wagner, Arnika K
Kadri, Nadir
Tibbitt, Chris
van de Ven, Koen
Bagawath-Singh, Sunitha
Oliinyk, Denys
LeGresley, Eric
Campbell, Nicole
Trittel, Stephanie
Riese, Peggy
Citations
Altmetric:
Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2022-09-16
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
PD-1 expression on mouse intratumoral NK cells and its effects on NK cell phenotype.
Translated Title
Published in
iScience 2022;25(10):105137
Abstract
Although PD-1 was shown to be a hallmark of T cells exhaustion, controversial studies have been reported on the role of PD-1 on NK cells. Here, we found by flow cytometry and single cell RNA sequencing analysis that PD-1 can be expressed on MHC class I-deficient tumor-infiltrating NK cells in vivo. We also demonstrate distinct alterations in the phenotype of PD-1-deficient NK cells and a more mature phenotype which might reduce their capacity to migrate and kill in vivo. Tumor-infiltrating NK cells that express PD-1 were highly associated with the expression of CXCR6. Furthermore, our results demonstrate that PD-L1 molecules in membranes of PD-1-deficient NK cells migrate faster than in NK cells from wild-type mice, suggesting that PD-1 and PD-L1 form cis interactions with each other on NK cells. These data demonstrate that there may be a role for the PD-1/PD-L1 axis in tumor-infiltrating NK cells in vivo.