Methodiekontwikkeling voor een bioassay met nematoden in natuurlijke grond
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Series / Report no.
Open Access
Type
Report
Language
nl
Date
1994-04-30
Research Projects
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Journal Issue
Title
Methodiekontwikkeling voor een bioassay met
nematoden in natuurlijke grond
Translated Title
Development of a method for a bioassay with
nematodes in natural soil
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Abstract
Abstract niet beschikbaar
This report describes the experiments that were carried out, to develop a bioassay with nematodes in a polluted natural soil. The nematode Plectus acuminatus, was cultured in 0.5% sloppy-agar with Acinetobacter spec. as a food source. For the experiments small plastic pots were used with 10 g soil polluted with heavy metals, from the Budel-area. One hundred nematodes were inoculated, and exposed for 7 days in a climate-chamber by 20 degrees C in the dark. In the first experiment with three levels of heavy metal contamination and pH-adjustment, the recovery of nematodes was very low (maximum 22%). The results showed that nematodes can survive in experiments with natural soil. Also a weak trend was found: more nematodes were caught back in the clean soil. Liming (to adjust pH) did not seem to have an effect on survival. To optimize the survival a second experiment was carried out. Several methodical items were investigated (Liming, Extraction method, Food, Injection of nematodes). The results show that the survival percentage can be optimized to an average of 79%. Additionally, the possibilities to preserve Plectus acuminatus in a frozen state were investigated, in order to use identical genetical material in future experiments. It appeared to be possible to revive nematodes after freezing at -70 C degrees. The highest survival (19%), after 7 days at -70 degrees C, was found for nematodes in pure tap water.
This report describes the experiments that were carried out, to develop a bioassay with nematodes in a polluted natural soil. The nematode Plectus acuminatus, was cultured in 0.5% sloppy-agar with Acinetobacter spec. as a food source. For the experiments small plastic pots were used with 10 g soil polluted with heavy metals, from the Budel-area. One hundred nematodes were inoculated, and exposed for 7 days in a climate-chamber by 20 degrees C in the dark. In the first experiment with three levels of heavy metal contamination and pH-adjustment, the recovery of nematodes was very low (maximum 22%). The results showed that nematodes can survive in experiments with natural soil. Also a weak trend was found: more nematodes were caught back in the clean soil. Liming (to adjust pH) did not seem to have an effect on survival. To optimize the survival a second experiment was carried out. Several methodical items were investigated (Liming, Extraction method, Food, Injection of nematodes). The results show that the survival percentage can be optimized to an average of 79%. Additionally, the possibilities to preserve Plectus acuminatus in a frozen state were investigated, in order to use identical genetical material in future experiments. It appeared to be possible to revive nematodes after freezing at -70 C degrees. The highest survival (19%), after 7 days at -70 degrees C, was found for nematodes in pure tap water.
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