Isolation of Listeria monocytogenes from reference materials, BCR-FOOD Trial 3
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Series / Report no.
Open Access
Type
Report
Language
en
Date
1991-03-31
Research Projects
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Journal Issue
Title
Isolation of Listeria monocytogenes from reference
materials, BCR-FOOD Trial 3
Translated Title
Isolatie van Listeria monocytogenes in
referentiematerialen, BCR-FOOD Trial 3
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Abstract
Abstract niet beschikbaar
Reference materials containing Listeria monocytogenes were tested in a collaborative study held in june 1989. Fourty laboratories participated. Each laboratory examinated 25 samples by 2 methods. For each method 5 of the 25 test samples were negative control samples (negative for Listeria). One method was standardized and used by all laboratories (Standard Method), the other was a method chosen by participating laboratory (OWN method). The median contamination level of the capsules was 9.27. Based on a lognormal distribution at least 19 out of 20 isolations should be made. With the Standard Method 97% of the capsules were found positive and 85% of the laboratories made at least 19 isolations. The OWN method gave significantly less isolations ; 85% of the capsules were found positive and 55% of the laboratories made at least 19 isolations. Four laboratories (10%) reported false positive isolations with the negative control samples. Concluded was that the Listeria monocytogenes reference materials are suitable for testing laboratory performance.
Reference materials containing Listeria monocytogenes were tested in a collaborative study held in june 1989. Fourty laboratories participated. Each laboratory examinated 25 samples by 2 methods. For each method 5 of the 25 test samples were negative control samples (negative for Listeria). One method was standardized and used by all laboratories (Standard Method), the other was a method chosen by participating laboratory (OWN method). The median contamination level of the capsules was 9.27. Based on a lognormal distribution at least 19 out of 20 isolations should be made. With the Standard Method 97% of the capsules were found positive and 85% of the laboratories made at least 19 isolations. The OWN method gave significantly less isolations ; 85% of the capsules were found positive and 55% of the laboratories made at least 19 isolations. Four laboratories (10%) reported false positive isolations with the negative control samples. Concluded was that the Listeria monocytogenes reference materials are suitable for testing laboratory performance.
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VHI
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