Validation of Microbiological Methods. Enumeration of Bacillus cereus according to ISO 7932 (second edition, 1993)
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Type
Report
Language
en
Date
1998-07-15
Research Projects
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Journal Issue
Title
Validation of Microbiological Methods. Enumeration
of Bacillus cereus according to ISO 7932 (second edition,
1993)
Translated Title
Validatie van microbiologische methoden. Telling
van Bacillus cereus volgens ISO 7932 (tweede editie,
1993)
Published in
Abstract
De Bacillus cereus methode, zoals beschreven in ISO
7932 (tweede editie, 1993), is gevalideerd in opdracht van de Europese
Commissie (Standards, Measurement and Testing Program). Kaas, vlees,
gedroogd aardappelpoeder en een referentiemateriaal zijn door 20 laboratoria
uit 17 landen in Europa en de USA onderzocht. Naast MEYP is in dit
onderzoek ook PEMBA medium meegenomen om een mogelijk verschil in resultaten
te detecteren. De herhaalbaarheid en reproduceerbaarheid bleken niet
gerelateerd te zijn aan het type voedingsmiddel of de concentratie B. cereus
in het monster. Voor de voedingsmiddelen werd een algemene herhaalbaarheid
van 0.29 log10 eenheden (verschilwaarde) of 2.0 (ratio) gevonden, en voor
de reproduceerbaarheid was dit 0.42 log10 eenheden (verschilwaarde) of 2.6
(ratio). Voor het referentiemateriaal was de herhaalbaarheid 0.11 log10
eenheden (verschilwaarde) of 1.3 (ratio) en de reproduceerbaarheid 0.22
log10 eenheden (verschilwaarde) of 1.7 (ratio). MEYP en PEMBA gaven
vergelijkbare resultaten.
The Bacillus cereus method, described in ISO 7932 (second edition, 1993), was validated by order of the European Commission (Standards, Measurement and Testing Program). Cheese, meat, dried potato and a reference material were examined by 20 laboratories in 17 countries in Europe and the USA. PEMBA medium was included along with MEYP in the study to detect possible differences in performance. Repeatability and reproducibility values appeared non-related to the type of food or the concentration of B. cereus present in the test sample. The general repeatability value found for the food samples was 0.29 log10 units (difference) or 2.0 (ratio), and for reproducibility, this was 0.42 log10 units (difference) or 2.6 (ratio). For the reference material, the repeatability was 0.11 log10 units(difference) or 1.3 (ratio) and the reproducibility, 0.22 log10 units (difference) or 1.7 (ratio). MEYP and PEMBA showed similar performance.
The Bacillus cereus method, described in ISO 7932 (second edition, 1993), was validated by order of the European Commission (Standards, Measurement and Testing Program). Cheese, meat, dried potato and a reference material were examined by 20 laboratories in 17 countries in Europe and the USA. PEMBA medium was included along with MEYP in the study to detect possible differences in performance. Repeatability and reproducibility values appeared non-related to the type of food or the concentration of B. cereus present in the test sample. The general repeatability value found for the food samples was 0.29 log10 units (difference) or 2.0 (ratio), and for reproducibility, this was 0.42 log10 units (difference) or 2.6 (ratio). For the reference material, the repeatability was 0.11 log10 units(difference) or 1.3 (ratio) and the reproducibility, 0.22 log10 units (difference) or 1.7 (ratio). MEYP and PEMBA showed similar performance.
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European Commission
Brussels
Brussels