A spitting image: molecular diagnostics applied to saliva enhance detection of and pneumococcal serotype carriage.
Miellet, Willem R van Veldhuizen, Janieke Litt, David Mariman, Rob Wijmenga-Monsuur, Alienke J Nieuwenhuijsen, Tessa Christopher, Jennifer Thombre, Rebecca Eletu, Seyi Bosch, Thijs
Miellet, Willem R
van Veldhuizen, Janieke
Litt, David
Mariman, Rob
Wijmenga-Monsuur, Alienke J
Nieuwenhuijsen, Tessa
Christopher, Jennifer
Thombre, Rebecca
Eletu, Seyi
Bosch, Thijs
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Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2023-04-17
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
A spitting image: molecular diagnostics applied to saliva enhance detection of and pneumococcal serotype carriage.
Translated Title
Published in
Front Microbiol 2023;14:1156695
Abstract
Quantitative PCR (qPCR)-based methods were applied to detect pneumococcus and pneumococcal serotypes in 971 saliva samples collected from 653 toddlers and 318 adults. Results were compared with culture-based and qPCR-based detection in nasopharyngeal samples collected from children and in nasopharyngeal and oropharyngeal samples collected from adults. Optimal C q cut-offs for positivity in qPCRs were determined via receiver operating characteristic curve analysis and accuracy of different approaches was assessed using a composite reference for pneumococcal and for serotype carriage based on isolation of live pneumococcus from the person or positivity of saliva samples determined with qPCR. To evaluate the inter-laboratory reproducibility of the method, 229 culture-enriched samples were tested independently in the second center.