A spitting image: molecular diagnostics applied to saliva enhance detection of and pneumococcal serotype carriage.
Miellet, Willem R; van Veldhuizen, Janieke; Litt, David; Mariman, Rob; Wijmenga-Monsuur, Alienke J; Nieuwenhuijsen, Tessa; Christopher, Jennifer; Thombre, Rebecca; Eletu, Seyi; Bosch, Thijs; Rots, Nynke Y; van Houten, Marianne Alice; Miller, Elizabeth; Fry, Norman K; Sanders, Elisabeth A M; TrzciĆski, Krzysztof
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Open Access
Type
Article
Language
en
Date
2023-04-17
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Title
A spitting image: molecular diagnostics applied to saliva enhance detection of and pneumococcal serotype carriage.
Translated Title
Published in
Front Microbiol 2023;14:1156695
Abstract
Quantitative PCR (qPCR)-based methods were applied to detect pneumococcus and pneumococcal serotypes in 971 saliva samples collected from 653 toddlers and 318 adults. Results were compared with culture-based and qPCR-based detection in nasopharyngeal samples collected from children and in nasopharyngeal and oropharyngeal samples collected from adults. Optimal C q cut-offs for positivity in qPCRs were determined via receiver operating characteristic curve analysis and accuracy of different approaches was assessed using a composite reference for pneumococcal and for serotype carriage based on isolation of live pneumococcus from the person or positivity of saliva samples determined with qPCR. To evaluate the inter-laboratory reproducibility of the method, 229 culture-enriched samples were tested independently in the second center.