Characterization of immunoaffinity chromatography materials for the determination of beta-agonists in biological samples
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Series / Report no.
Open Access
Type
Report
Language
en
Date
1994-04-30
Research Projects
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Journal Issue
Title
Characterization of immunoaffinity chromatography
materials for the determination of beta-agonists in biological
samples
Translated Title
[Karakterisering van immunoaffiniteits
chromatografie materialen voor de bepaling van beta-agonisten in biologische
materialen.]
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Abstract
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Chromatographic immunoaffinity materials against beta-agonists were prepared and validated. Specific immunochemical activity was determined for clenbuterol and was 61 +- 6 ng/mg gel. After one year of storage at 4 degrees C there was no significant reduction of the capacity. Under conditions of maximum binding the recovery for clenbuterol, salbutamol, terbutaline, cimaterol and mabuterol at a level of 200 ng, ranges from 83 to 96% with no significant changes after one year of storage.
Chromatographic immunoaffinity materials against beta-agonists were prepared and validated. Specific immunochemical activity was determined for clenbuterol and was 61 +- 6 ng/mg gel. After one year of storage at 4 degrees C there was no significant reduction of the capacity. Under conditions of maximum binding the recovery for clenbuterol, salbutamol, terbutaline, cimaterol and mabuterol at a level of 200 ng, ranges from 83 to 96% with no significant changes after one year of storage.
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EG/12