Transferability and reproducibility of exposed air-liquid interface co-culture lung models.
Braakhuis, Hedwig M; Gremmer, Eric R; Bannuscher, Anne; Drasler, Barbara; Keshavan, Sandeep; Rothen-Rutishauser, Barbara; Birk, Barbara; Verlohner, Andreas; Landsiedel, Robert; Meldrum, Kirsty; Doak, Shareen H; Clift, Martin J D; Erdem, Johanna Samulin; Foss, Oda A H; Zienolddiny-Narui, Shanbeh; Serchi, Tommaso; Moschini, Elisa; Weber, Pamina; Burla, Sabina; Kumar, Pramod; Schmid, Otmar; Zwart, Edwin; Vermeulen, Jolanda P; Vandebriel, Rob J
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Series / Report no.
Open Access
Type
Article
Language
en
Date
2023-05-18
Research Projects
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Journal Issue
Title
Transferability and reproducibility of exposed air-liquid interface co-culture lung models.
Translated Title
Published in
NanoImpact 2023; 31:100466
Abstract
Overall, the results of the 7 participating laboratories are quite similar. After exposing Calu-3 alone and Calu-3 co-cultures with macrophages, no effects of lipopolysaccharide (LPS), quartz (DQ12) or titanium dioxide (TiO2) NM-105 particles on the cell viability and barrier integrity were detected. LPS exposure induced moderate cytokine release in the Calu-3 monoculture, albeit not statistically significant in most labs. In the co-culture models, most laboratories showed that LPS can significantly induce cytokine release (IL-6, IL-8 and TNF-α). The exposure to quartz and TiO2 particles did not induce a statistically significant increase in cytokine release in both cell models probably due to our relatively low deposited doses, which were inspired by in vivo dose levels. The intra- and inter-laboratory comparison study indicated acceptable interlaboratory variation for cell viability/toxicity (WST-1, LDH) and transepithelial electrical resistance, and relatively high inter-laboratory variation for cytokine production.