The Chrono-Vax trial protocol: The effect of the time of day of influenza vaccination on immune responses in adults aged 60-85 years
Vink, Koen Brouwers, Tamara MJ Beckers, Lisa Smit, Debbie Vos, Martijn Guichelaar, Teun Bonten, Tobias N Kervezee, Laura van Baarle, Debbie Wallinga, Jacco
Vink, Koen
Brouwers, Tamara MJ
Beckers, Lisa
Smit, Debbie
Vos, Martijn
Guichelaar, Teun
Bonten, Tobias N
Kervezee, Laura
van Baarle, Debbie
Wallinga, Jacco
Series / Report no.
Open Access
Type
Journal Article
Article
Article
Language
en
Date of publication
2025-07-31
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
The Chrono-Vax trial protocol: The effect of the time of day of influenza vaccination on immune responses in adults aged 60-85 years
Translated Title
Published in
Contemp Clin Trials 2025; 156:108040
Abstract
Increasing evidence suggests that timing of vaccine administration affects immune responses, with some studies indicating that morning administration of the influenza vaccine elicits a stronger antibody response than afternoon vaccination in older adults. Existing trials focused on antibody responses, contrasting two time groups (morning versus afternoon), without assessing other immunological parameters, such as T-cell responses, which also play a crucial role in immunity to respiratory viruses and may contribute to the time-of-day dependent response to vaccination. Therefore, the Chrono-Vax trial aims to determine the effect of influenza vaccination timing on antibody and T-cell responses across a continuous time window from 09:00-17:00 to gain novel insights into an optimal administration time. Additionally, this study will investigate how chronotype influences the relationship between vaccination timing and vaccine-induced immune responses and examine the impact of vaccination timing on the incidence of influenza-like illness (ILI) and influenza infection up to six months post-vaccination. A total of 440 adults aged 60-85 years will be included in the trial and receive the seasonal influenza vaccine at a random time between 09:00 and 17:00. Blood samples will be collected at baseline and 28 days post-vaccination to determine vaccine-induced immune responses. ILI symptoms and influenza infection will be monitored by participants up to six months after vaccination using a diary and rapid diagnostic self-tests, respectively. As such, the Chrono-Vax trial aims to determine the optimal time of day for influenza vaccination, thereby contributing to more effective vaccination strategies and increased protection against severe disease in older adults.