Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains - A case example
van der Gaag, Kristiaan J van Marion, Vincent van den Berg, Redmar R Weiler, Natalie EC Hoogenboom, Jerry Kal, Arnoud Kayser, Manfred de Knijff, Peter Laros, Jeroen FJ Sijen, Titia
van der Gaag, Kristiaan J
van Marion, Vincent
van den Berg, Redmar R
Weiler, Natalie EC
Hoogenboom, Jerry
Kal, Arnoud
Kayser, Manfred
de Knijff, Peter
Laros, Jeroen FJ
Sijen, Titia
Series / Report no.
Open Access
Type
Journal Article
Case Reports
Article
Case Reports
Article
Language
en
Date of publication
2025-05-03
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains - A case example
Translated Title
Published in
Forensic Sci Int Genet 2025; 78:103292
Abstract
In forensic casework, monozygotic twins have always provided a challenge, as routinely used forensic Short Tandem Repeat (STR) profiles are not able to differentiate between the twin individuals. In this study, we applied a method to discriminate between two monozygotic twin brothers in a sexual assault case that is unique and challenging for several reasons: the use of contact stains as evidence, the stains contain DNA from two persons (victim and one of the brothers), have minimal amounts of DNA, and there are PCR inhibiting factors. Despite these challenging factors, we present a successfully solved case in which whole genome sequencing was applied to identify multiple somatic differences between the two brothers. Validation of the developed methods and the identified differences was performed on material provided by the two siblings, before applying the method on two evidentiary stains. A statistical framework was developed to provide a likelihood calculation for this type of analysis in mixed stains. The results were accepted in court and contributed to the conviction of the case suspect. Here we provide the scientific details in order to encourage the use of this approach in more such cases in the future.