Electrospray ionisatie massaspectrometrie: Verkenning van het toepassingsgebied
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Series / Report no.
Open Access
Type
Report
Language
nl
Date
1996-04-30
Research Projects
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Journal Issue
Title
Electrospray ionisatie massaspectrometrie:
Verkenning van het toepassingsgebied
Translated Title
Electrospray ionization mass spectrometry.
Exploration of the applicability
Published in
Abstract
Betreft de resultaten van een studie naar de
toepasbaarheid van electrospray ionisatie massaspectrometrie (ESI-MS). Er
werd onderzoek verricht op het gebied van de eitwit- en peptide-analyse en
de bepaling van polaire bestrijdingsmiddelen, macrolide antibiotica en
metabolieten van polycyclische aromatische koolwaterstoffen. De techniek
werd zowel met continue infusie van peptide- en eiwitoplossingen toegepast
als in combinatie met HPLC voor de bepaling van laag-moleculaire
verbindingen. De molecuulmassa van enkele eiwitten (17 - 66 kDa) en
peptiden (5 kDa) kon met grote nauwkeurigheid bepaald worden.
Bestrijdingsmiddelen en macrolide antibiotica werden op laag
concentratieniveau bepaald met behulp van HPLC-ESI-MS. De identificatie van
een naftaleenmetaboliet gaf de mogelijkheden van ESI-MS aan bij de
structuuropheldering.
The results of a study into the applicability of electrospray ionization mass spectrometry (ESI-MS) have been described. The potential of the technique for protein and peptide analysis, for structure elucidation of polynuclear aromatic hydrocarbons and for the detection of polar pesticides and macrolide antibiotics, was investigated. ESI-MS was employed with continuous infusion of solutions of peptides and proteins and combined with HPLC for the analysis of low-molecular-weight compounds. Molecular weight determinations of 17 - 66 kDa proteins and 5 kDa peptides were achieved with high accuracy. Pesticides and macrolide antibiotics were determined at low concentration levels by HPLC-ESI-MS. The identification of a metabolite of naphthalene showed the potential of ESI-MS for structure elucidation.
The results of a study into the applicability of electrospray ionization mass spectrometry (ESI-MS) have been described. The potential of the technique for protein and peptide analysis, for structure elucidation of polynuclear aromatic hydrocarbons and for the detection of polar pesticides and macrolide antibiotics, was investigated. ESI-MS was employed with continuous infusion of solutions of peptides and proteins and combined with HPLC for the analysis of low-molecular-weight compounds. Molecular weight determinations of 17 - 66 kDa proteins and 5 kDa peptides were achieved with high accuracy. Pesticides and macrolide antibiotics were determined at low concentration levels by HPLC-ESI-MS. The identification of a metabolite of naphthalene showed the potential of ESI-MS for structure elucidation.
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