Multicentre evaluation of a selective isolation protocol for detection of mcr-positive E. coli and Salmonella spp. in food-producing animals and meat.
Perrin-Guyomard, Agnès Granier, Sophie A Slettemeås, Jannice Schau Anjum, Muna Randall, Luke AbuOun, Manal Pauly, Natalie Irrgang, Alexandra Hammerl, Jens Andre Kjeldgaard, Jette Sejer
Perrin-Guyomard, Agnès
Granier, Sophie A
Slettemeås, Jannice Schau
Anjum, Muna
Randall, Luke
AbuOun, Manal
Pauly, Natalie
Irrgang, Alexandra
Hammerl, Jens Andre
Kjeldgaard, Jette Sejer
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Open Access
Type
Article
Language
en
Date
2022-04-07
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Title
Multicentre evaluation of a selective isolation protocol for detection of mcr-positive E. coli and Salmonella spp. in food-producing animals and meat.
Translated Title
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Letters in applied microbiology 2022; 75(2):224-233
Abstract
This study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive E. coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving twelve laboratories from nine European countries. All participants applied the same methodology combining a multiplex PCR performed on DNA extracted from a pre-enrichment step, followed by a selective culture step on three commercially-available chromogenic agar-plates. The test panel was composed of two negative samples and four samples artificially contaminated with E. coli and Salmonella spp. respectively harbouring mcr-1 or mcr-3 and mcr-4 or mcr-5 genes. PCR screening resulted in a specificity of 100% and a sensitivity of 83%. Sensitivity of each agar medium to detect mcr-positive colistin-resistant E. coli or Salmonella spp. strains was 86% for CHROMID® Colistin R, 75% for CHROMagarTM COL-APSE and 70% for COLISTIGRAM. This combined method was effective to detect and isolate most of the E. coli or Salmonella spp. strains harbouring different mcr genes from food-producing animals and food products and might thus be used as a harmonized protocol for the screening of mcr genes in food-producing animals and food products in Europe.