Invloed van matrix en eluens op de sporenanalyse van enkele anionen met ionchromatografie
Neele J Cleven RFMJ Wiel HJ van de
Neele J
Cleven RFMJ
Wiel HJ van de
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Series / Report no.
Open Access
Type
Report
Language
nl
Date of publication
1997-03-31
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
Invloed van matrix en eluens op de sporenanalyse van
enkele anionen met ionchromatografie
Translated Title
Influence of matrix and eluent on the analysis of
trace anions with ion chromatography
Published in
Abstract
De mate van beinvloeding van diverse matrices op de
resultaten van een ionchromatografische methode voor de bepaling van onder
andere chloride, nitraat en sulfaat is onderzocht. De berekende overspraak
van de methode is nihil. De variatiecoefficient voor 2 mumol/l nitraat en
sulfaat is 2%. Onbehandelde monsters kunnen leiden tot een snel vervuilde
guardkolom, een hogere pompdruk en een sulfaatniveau van 0,5 mumol/l in
blanco's. De methode bleek gevoelig voor sterke ionogene matrices. In deze
matrices elueren naast sulfaat ook chloride en nitraat als verontreinigingen
van het eluens. Bij hoge anionenconcentraties in monsters is snel een 'on
column eluent change' effect bereikt. Deze hoge monstermatrices beinvloeden
de retentietijden, door verbreding de piekoppervlak/hoogteverhouding en de
analytische respons van sporen van anionen. Redu- ceren van deze hoge
ionsterkten door verdunning of ionenuitwisseling in de monstermatrix
voorkomt fouten in de identificering en in de kwantificering van
meetresultaten.
The impact of several matrices on results of the ionchromatographic method for the determination of chloride, nitrate en sulphate in water is demonstrated. Carry-over of the method is negligible. The RSD for both 2 mumol/l nitrate and sulphate is 2%. Untreated samples can lead to a quick polluted guardcolumn, increased pump pressure and a sulphate level of 0,5 mumol/l in blanks. The method is sensitive for high ionic matrices. In those matrices, which differ strongly from the low carbonate/ bicarbonate buffer eluent, elution of the chloride, nitrate and sulphate from impurities of the eluent takes place. In the high ionic matrices the 'on column eluent change' effect is reached rapidly. High sample matrix composition affects the retention process in the column, the area/height values (broadening) of the peak and the analytical response of trace anions. Reduction of high ionic matrices by dillution or ion exchange of the sample matrix prevents errors in identification and quantitation of measurements.
The impact of several matrices on results of the ionchromatographic method for the determination of chloride, nitrate en sulphate in water is demonstrated. Carry-over of the method is negligible. The RSD for both 2 mumol/l nitrate and sulphate is 2%. Untreated samples can lead to a quick polluted guardcolumn, increased pump pressure and a sulphate level of 0,5 mumol/l in blanks. The method is sensitive for high ionic matrices. In those matrices, which differ strongly from the low carbonate/ bicarbonate buffer eluent, elution of the chloride, nitrate and sulphate from impurities of the eluent takes place. In the high ionic matrices the 'on column eluent change' effect is reached rapidly. High sample matrix composition affects the retention process in the column, the area/height values (broadening) of the peak and the analytical response of trace anions. Reduction of high ionic matrices by dillution or ion exchange of the sample matrix prevents errors in identification and quantitation of measurements.
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