External validation of WGS-based antimicrobial susceptibility prediction tools, KOVER-AMR and ResFinder 4.1 for Escherichia coli clinical isolates.
Verschuuren, T D Bosch, T Mascaro, V Willems, R J L Kluytmans, J A J W
Verschuuren, T D
Bosch, T
Mascaro, V
Willems, R J L
Kluytmans, J A J W
Citations
Altmetric:
Series / Report no.
Open Access
Type
Article
Language
en
Date of publication
2022-06-01
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
External validation of WGS-based antimicrobial susceptibility prediction tools, KOVER-AMR and ResFinder 4.1 for Escherichia coli clinical isolates.
Translated Title
Published in
Clinical microbiology and infection 2022; 28(11):1465-1470
Abstract
A random sample of 234 E. coli, and 283 3rd generation cephalosporin-resistant E. coli isolates from urine and blood were collected (2014-17). Culture-AST was performed using VITEK 2 and BD Phoenix. Sequences were used as input for KOVER-AMR-SCM, KOVER-AMR-CART and ResFinder 4.1. The concordance, major error rate (MER), and very major error rate (VMER) were calculated, with subsequent comparison to U.S. Food and Drug Administration (FDA) criteria (MER ≤3%, and VMER with a 95% confidence interval (CI) ≤1.5%-≤7.5%).