Protocol for microbial profiling of low-biomass upper respiratory tract samples
Odendaal, Mari-Lee Chu, Mei Ling JN Arp, Kayleigh van Beveren, Gina J Biesbroek, Giske Binkowska, Justyna Bosch, Thijs Groot, James A Hasrat, Raiza Kuiling, Sjoerd
Odendaal, Mari-Lee
Chu, Mei Ling JN
Arp, Kayleigh
van Beveren, Gina J
Biesbroek, Giske
Binkowska, Justyna
Bosch, Thijs
Groot, James A
Hasrat, Raiza
Kuiling, Sjoerd
Series / Report no.
Open Access
Type
Journal Article
Article
Article
Language
en
Date of publication
2025-04-07
Year of publication
Research Projects
Organizational Units
Journal Issue
Title
Protocol for microbial profiling of low-biomass upper respiratory tract samples
Translated Title
Published in
STAR Protoc 2025; 6(2):103740
Abstract
The upper respiratory tract (URT) microbiota plays a role in both acute and chronic respiratory health outcomes and consists of multiple ecologically distinct niches, all of which have low bacterial biomass. Here, we present a protocol for microbial profiling of low-biomass URT samples. We describe steps for collecting, storing, and extracting DNA. We then detail procedures for performing 16S rRNA sequencing, using an Illumina MiSeq platform, to characterize microbial communities. For complete details on the use and execution of this protocol, please refer to Odendaal et al..