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Eleventh CRL-Salmonella interlaboratory comparison study (2006) on typing of Salmonella spp.
Berk PA Maas HME de Pinna E Mooijman KA
Berk PA
Maas HME
de Pinna E
Mooijman KA
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Series / Report no.
RIVM rapport 330604001
Open Access
Type
Report
Language
en
Date of publication
2006-09-28
Year of publication
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330604001.pdf
Adobe PDF, 402.92 KB
Research Projects
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Journal Issue
Title
Eleventh CRL-Salmonella interlaboratory comparison study (2006) on typing of Salmonella spp.
Translated Title
Elfde CRL-Salmonella ringonderzoek (2006) voor de typering van Salmonella spp.
Published in
Abstract
Het elfde ringonderzoek voor de typering van Salmonella werd in maart 2006 georganiseerd door het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella, Bilthoven, Nederland) in samenwerking met de Health Protection Agency (HPA, Londen, Verenigd Koninkrijk). 26 Nationale Referentie Laboratoria voor Salmonella (NRLs-Salmonella) inclusief Noorwegen en 31 Enter-Net Laboratoria (ENLs), waarvan 3 ook NRL, namen deel aan de studie. 20 Stammen van species Salmonella enterica subspecies enterica werden geselecteerd voor de serotypering. Tien stammen van Salmonella Enteritidis (SE) en 10 stammen van Salmonella Typhimurium (STM) werden geselecteerd voor faagtypering. In het algemeen werden geen problemen gevonden met de typering van de O-antigenen. 98 % procent van de NRLs en 98 % van de ENLs typeerden de O-antigenen correct. Slechts enkele laboratoria hadden problemen met het typeren van de H-antigenen. De H-antigenen werden correct getypeerd door 94 % van de NRLs en door 94 % van de ENLs. 93 % procent van de NRLs en 93 % van de ENLs gaven de 20 serotyperingsstammen de goede serovar naam. De meeste NRLs vonden goede resultaten met de faagtypering. De zeven NRLs faagtypeerden 94 % van de Salmonella Enteritidis stammen correct en 99 % van de Salmonella Typhimurium stammen. De achttien ENLs hebben 84 % van de Salmonella Enteritidis en 89 % van de Salmonella Typhimurium stammen goed gefaagtypeerd.
The eleventh interlaboratory comparison study on the typing of Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Health Protection Agency (HPA, London, United Kingdom) in March 2006. 26 National Reference Laboratories for Salmonella (NRLs-Salmonella), including Norway, and 31 Enter-Net Laboratories (ENLs), three of which also NRLs, participated in the study. In total, 20 strains of the species Salmonella enterica subspecies enterica were selected for serotyping. 10 strains of Salmonella Enteritidis (SE) and 10 strains of Salmonella Typhimurium (STM) were selected for phage typing. In general, no problems were encountered with the typing of the O-antigens. 98 % of the NRLs and 98 % of the ENLs were able to correctly type the O-antigens. A few laboratories had problems typing the H-antigens. The H-antigens were typed correctly by 94 % of the NRLs and by 94 % of the ENLs. 93 % of the NRLs and 93 % of the ENLs indicated correct serovar names for the 20 serotyping strains. The phage typing results of the majority of the NRLs were found to be good. The seven NRLs phage typed 94 % of the Salmonella Enteritidis strains correct and 99 % of the Salmonella Typhimurium strains. 18 ENLs participated in the phagetyping. The Salmonella Enteritidis strains were correctly phage typed by 84 % of the ENLs and Salmonella Typhimurium by 89 % of the ENLs.
The eleventh interlaboratory comparison study on the typing of Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Health Protection Agency (HPA, London, United Kingdom) in March 2006. 26 National Reference Laboratories for Salmonella (NRLs-Salmonella), including Norway, and 31 Enter-Net Laboratories (ENLs), three of which also NRLs, participated in the study. In total, 20 strains of the species Salmonella enterica subspecies enterica were selected for serotyping. 10 strains of Salmonella Enteritidis (SE) and 10 strains of Salmonella Typhimurium (STM) were selected for phage typing. In general, no problems were encountered with the typing of the O-antigens. 98 % of the NRLs and 98 % of the ENLs were able to correctly type the O-antigens. A few laboratories had problems typing the H-antigens. The H-antigens were typed correctly by 94 % of the NRLs and by 94 % of the ENLs. 93 % of the NRLs and 93 % of the ENLs indicated correct serovar names for the 20 serotyping strains. The phage typing results of the majority of the NRLs were found to be good. The seven NRLs phage typed 94 % of the Salmonella Enteritidis strains correct and 99 % of the Salmonella Typhimurium strains. 18 ENLs participated in the phagetyping. The Salmonella Enteritidis strains were correctly phage typed by 84 % of the ENLs and Salmonella Typhimurium by 89 % of the ENLs.
Description
Publisher
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Sponsors
EC; Legislation Veterinaire et Zootechnique