Bacteriologisch en serologisch onderzoek van Helicobacter species bij laboratoriummuizen
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Series / Report no.
Open Access
Type
Report
Language
nl
Date
1998-11-27
Research Projects
Organizational Units
Journal Issue
Title
Bacteriologisch en serologisch onderzoek van
Helicobacter species bij laboratoriummuizen
Translated Title
Bacteriological and serological investigation of
Helicobacter species in laboratory mice
Published in
Abstract
Helicobacter soorten zijn spiraalvormige Gramnegatieve
bacterien die voorkomen in het maagdarmkanaal van mens en dier.
Helicobacter hepaticus koloniseert bij muizen o.a. het ileum en het cecum
en is bij meerdere muizenstammen geassocieerd met chronische actieve
hepatitis en bij de A/J stam met de ontwikkeling van levertumoren. Het
opkweken van gevriesdroogde Helicobacter stammen bleek lastiger dan de kweek
uit feces en organen. Isolaten konden m.b.v. het Grampeparaat,
biochemische testen en analyse van celwandlipiden redelijk goed als
Helicobacter worden geklasseerd, maar benoeming tot op de soort bleek minder
makkelijk. Er werd een ELISA opgezet voor de bepaling van antistoffen bij
muizen tegen whole cell antigeen van H. hepaticus. Er werd vrijwel geen
kruisreactiviteit gevonden met andere Helicobacters of bacterien uit andere
families. De bruikbaarheid van de ELISA werd onderzocht bij BALB/c en N:NIH
muizen die oraal en op natuurlijke wijze werden besmet met H.hepaticus. De
NIH muizen vertoonden een betere antistofrespons dan de BALB/c muizen.
Zowel uit oraal geinfecteerde als op natuurlijke wijze besmette muizen van
beide stammen werd H. hepaticus teruggekweekt. Bij de N:NIH muizen werd in
het ileum een lichte ontsteking van de darmvlokken gevonden. Tenslotte
werden populaties muizen onderzocht op besmetting met Helicobacter soorten
en op antistoffen tegen H. hepaticus. Gnotobiotische en SPF muizen bleken
niet besmet met Helicobacter sp. maar besmetting werd wel gevonden bij 2
conventionele transgene muizenstammen.
Helicobacter species are spiral shaped Gram negative bacteria that can be found in the gastrointestinal tract in humans and animals. Helicobacter hepaticus colonizes the ileum and cecum of various mouse strains and has in one mouse strain been associated with chronic active hepatitis and the development of neoplastic liver lesions. Culturing of freeze dried Helicobacter species appeared more difficult than primary isolation from animals. Using Gram stain, biochemical profiling and determination of cell wall lipids, isolates could be rather easily assigned to the genus Helicobacter, but speciation was more difficult. An ELISA was developed for measuring antibodies in mice to whole cell antigen of H. hepaticus. Antibodies raised against heterologous Helicobacter species and species of other bacterial genera did not clearly react with the antigen. The suitability of the ELISA was further evaluated in orally dosed and naturally infected BALB/c and N:NIH mice. N:NIH mice better seroconverted to H. hepaticus than BALB/c mice and the bacterium was recovered from orally infected and naturally infected animals of both mouse strains. N:NIH mice showed slight inflammation of the ileal villi. Finally, various mouse colonies were examined for Helicobacter infection by culture and serology. Gnotobiotic and SPF mice appeared uninfected but Helicobacter infection was diagnosed in 2 conventional transgenic mouse strains.
Helicobacter species are spiral shaped Gram negative bacteria that can be found in the gastrointestinal tract in humans and animals. Helicobacter hepaticus colonizes the ileum and cecum of various mouse strains and has in one mouse strain been associated with chronic active hepatitis and the development of neoplastic liver lesions. Culturing of freeze dried Helicobacter species appeared more difficult than primary isolation from animals. Using Gram stain, biochemical profiling and determination of cell wall lipids, isolates could be rather easily assigned to the genus Helicobacter, but speciation was more difficult. An ELISA was developed for measuring antibodies in mice to whole cell antigen of H. hepaticus. Antibodies raised against heterologous Helicobacter species and species of other bacterial genera did not clearly react with the antigen. The suitability of the ELISA was further evaluated in orally dosed and naturally infected BALB/c and N:NIH mice. N:NIH mice better seroconverted to H. hepaticus than BALB/c mice and the bacterium was recovered from orally infected and naturally infected animals of both mouse strains. N:NIH mice showed slight inflammation of the ileal villi. Finally, various mouse colonies were examined for Helicobacter infection by culture and serology. Gnotobiotic and SPF mice appeared uninfected but Helicobacter infection was diagnosed in 2 conventional transgenic mouse strains.
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