Uitbreiding second messengers: fosfoinositolsysteem. Eindrapportage over pilotexperimenten
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Series / Report no.
Open Access
Type
Report
Language
nl
Date
1994-01-31
Research Projects
Organizational Units
Journal Issue
Title
Uitbreiding second messengers: fosfoinositolsysteem. Eindrapportage over pilotexperimenten
Translated Title
[Extension of second messengers: phosphoinositolsystem. Final report on pilotexperiments.]
Published in
Abstract
A pilot study was conducted in order to investigate the possibility to measure the IP3 (inositoltrisphosphate) content in cardiovascular tissue of the rat, by means of a radioimmunoassay. With the kit is was possible to obtain a linear standard calibration curve. In extracts of control- and 10 mM NaF stimulated aorta's and left ventricles it appeared that with the applied method the measurement of IP3 was unsuccessful. In contrast to the sample preparation method, the measurement assay is not problematic. It was decided not to continue further investigations.<br>
Description
Publisher
Rijksinstituut voor Volksgezondheid en Milieu RIVM
Sponsors
RIVM