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High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
Aires-de-Sousa, Marta; Boye, K; Lencastre, H de; Deplano, A; Enright, M C; Etienne, J; Friedrich, A; Harmsen, D; Holmes, A; Huijsdens, Xander W; Kearns, A M; Mellmann, A; Meugnier, H; Rasheed, J K; Spalburg, Emile; Strommenger, B; Struelens, M J; Tenover, F C; Thomas, J; Vogel, U; Westh, H; Xu, J; Witte, W
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Open Access
Type
Article
Language
en
Date
2006-02-01
Research Projects
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Title
High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.
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Abstract
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.