Challenging the gold standard: the limitations of molecular assays for detection of Mycobacterium tuberculosis heteroresistance
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Open Access
Type
Article
Language
en
Date
2024-01-29
Research Projects
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Journal Issue
Title
Challenging the gold standard: the limitations of molecular assays for detection of Mycobacterium tuberculosis heteroresistance
Translated Title
Published in
Thorax 2024;79(7):670-675
Abstract
Heteroresistant infections are defined as infections in which a mixture of drug-resistant and drug-susceptible populations are present. In Mycobacterium tuberculosis (M. tb), heteroresistance poses a challenge in diagnosis and has been linked with poor treatment outcomes. We compared the analytical sensitivity of molecular methods, such as GeneXpert and whole genome sequencing (WGS) in detecting heteroresistance when compared with the 'gold standard' phenotypic assay: the agar proportion method (APM).