Coupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Exposed to MoS Nanosheets and Ionic Mo.

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dc.contributor.authorSun, Kailun
dc.contributor.authorWhite, Jason C
dc.contributor.authorQiu, Hao
dc.contributor.authorvan Gestel, Cornelis A M
dc.contributor.authorPeijnenburg, Willie J G M
dc.contributor.authorHe, Erkai
dc.date.accessioned2023-07-25T07:11:39Z
dc.date.available2023-07-25T07:11:39Z
dc.date.issued2023-07-20
dc.description.abstractMolybdenum disulfide (MoS2) nanosheets are increasingly applied in several fields, but effective and accurate strategies to fully characterize potential risks to soil ecosystems are lacking. We introduce a coelomocyte-based in vivo exposure strategy to identify novel adverse outcome pathways (AOPs) and molecular endpoints from nontransformed (NTMoS2) and ultraviolet-transformed (UTMoS2) MoS2 nanosheets (10 and 100 mg Mo/L) on the earthworm Eisenia fetida using nontargeted lipidomics integrated with transcriptomics. Machine learning-based digital pathology analysis coupled with phenotypic monitoring was further used to establish the correlation between lipid profiling and whole organism effects. As an ionic control, Na2MoO4 exposure significantly reduced (61.2-79.5%) the cellular contents of membrane-associated lipids (glycerophospholipids) in earthworm coelomocytes. Downregulation of the unsaturated fatty acid synthesis pathway and leakage of lactate dehydrogenase (LDH) verified the Na2MoO4-induced membrane stress. Compared to conventional molybdate, NTMoS2 inhibited genes related to transmembrane transport and caused the differential upregulation of phospholipid content. Unlike NTMoS2, UTMoS2 specifically upregulated the glyceride metabolism (10.3-179%) and lipid peroxidation degree (50.4-69.4%). Consequently, lipolytic pathways were activated to compensate for the potential energy deprivation. With pathology image quantification, we report that UTMoS2 caused more severe epithelial damage and intestinal steatosis than NTMoS2, which is attributed to the edge effect and higher Mo release upon UV irradiation. Our results reveal differential AOPs involving soil sentinel organisms exposed to different Mo forms, demonstrating the potential of liposome analysis to identify novel AOPs and furthermore accurate soil risk assessment strategies for emerging contaminants.en_US
dc.identifier.doi10.1021/acs.est.3c02518
dc.identifier.eissn1520-5851
dc.identifier.journalEnviron Sci Technol 2023; 57(30):11009-11021en_US
dc.identifier.pmid37471269
dc.identifier.urihttp://hdl.handle.net/10029/626855
dc.language.isoenen_US
dc.source.countryUnited States
dc.source.journaltitleEnvironmental science & technology
dc.subjectMoS2 nanosheetsen_US
dc.subjectcoelomocyteen_US
dc.subjectecological risken_US
dc.subjectliposomesen_US
dc.subjectmolecular mechanismsen_US
dc.subjectmulti-omicsen_US
dc.subjectultraviolet irradiationen_US
dc.titleCoupled Lipidomics and Digital Pathology as an Effective Strategy to Identify Novel Adverse Outcome Pathways in Exposed to MoS Nanosheets and Ionic Mo.en_US
dc.typeArticleen_US
dspace.entity.typePublication
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