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dc.contributor.authorArend, Sandra M
dc.contributor.authorHaas, Petra de
dc.contributor.authorLeyten, Eliane
dc.contributor.authorRosenkrands, Ida
dc.contributor.authorRigouts, Leen
dc.contributor.authorAndersen, Peter
dc.contributor.authorMijs, Wouter
dc.contributor.authorDissel, Jaap T van
dc.contributor.authorSoolingen, Dick van
dc.date.accessioned2007-03-20T13:24:59Z
dc.date.available2007-03-20T13:24:59Z
dc.date.issued2005-04-15
dc.identifier.citationJ. Infect. Dis. 2005, 191(8):1301-10en
dc.identifier.issn0022-1899
dc.identifier.pmid15776377
dc.identifier.doi10.1086/428950
dc.identifier.urihttp://hdl.handle.net/10029/10708
dc.description.abstractMycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.
dc.format.extent409173 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.titleESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii.en
dc.typeArticleen
dc.format.digYES
refterms.dateFOA2018-12-18T09:41:57Z
html.description.abstractMycobacterium kansasii consists of 5 genetically distinct groups, of which 2 are associated with human disease. Determinants of the differences in virulence are unknown. Potential genes of interest are esat-6 and cfp-10, which are associated with virulence of Mycobacterium tuberculosis and Mycobacterium bovis but are lacking in bacille Calmette-Guérin and in most environmental mycobacteria (M. kansasii is an exception). We investigated esat-6 and cfp-10 genes in 22 clinical and 14 environmental isolates of M. kansasii. Both were present in all isolates; each genetic group had its own characteristic Southern-blot pattern corresponding to a highly conserved fingerprint pattern. Nucleotide sequences of the genes differed 12.6% and 10.1%, respectively, from the M. tuberculosis homologues, but the deduced amino acid sequences were <5% different. In vitro, clinical and environmental genotypes of M. kansasii expressed CFP-10 and ESAT-6. Thus, virulence of M. kansasii is not directly related to esat-6 and cfp-10 genes or gene expression.


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