• Test results of Salmonella typing by the NRLs-Salmonella in the Member States of the EU and the EnterNet Laboratories - Collaborative study VI (2001) on typing of Salmonella

      Korver H; Raes M; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2002-09-06)
      Test results of Salmonella sero- and phage typing and antimicrobial susceptibility testing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories: Collaborative study VI (2001) for Salmonella. The sixth collaborative typing study for Salmonella was organised by the Community Reference Laboratory for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, UK. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENLs. The results of these three NRL-ENL laboratories will only be evaluated with the NRLs for Salmonella. In total, 19 strains of the species Salmonella enterica subsp. enterica and one strain of the species Salmonella enterica subsp. arizonae were selected for serotyping and antimicrobial susceptibility testing, while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Antimicrobial susceptibility testing revealed data showing that standardisation of this technique would be required to allow for comparison between laboratories. The majority of the EnterNet Laboratories and National Reference Laboratories for Salmonella did not encounter major problems with phage typing of STM and SE strains.
    • Test results of Salmonella typing by the National Reference Laboratories for Salmonella in the Member States of the European Union and the EnterNet Laboratories - Collaborative study VII (2002) on typing of Salmonella

      Korver H; Maas HME; Ward LR; Wannet WJB; Henken AM; MGB; LIS (PHLS-Colindale/London, 2003-02-13)
      A seventh collaborative typing study forry for Salmonella (CRL-Salmonella, Bilthoven, The Netherlands) in collaboration with the Public Health Laboratory Services (PHLS), London, United Kingdom. Seventeen National Reference Laboratories for Salmonella (NRLs-Salmonella) and 15 EnterNet laboratories (ENLs) participated in the study. Three of the NRLs for Salmonella are also ENL. The results obtained by these three NRL-ENL laboratories will only be evaluated in conjunction with results of the 17 NRLs- Salmonella. In total, 20 strains of the species Salmonella enterica subspecies enterica were selected for serotyping while 10 strains of Salmonella Typhimurium (STM) and 10 strains of Salmonella Enteritidis (SE) were selected for phage typing. In general, no problems were encountered with the typing of the O antigens. However, some laboratories had problems with typing the H antigens. Almost all laboratories faced only minor differences in phage typing. Some ENLs had considerable problems with the phage typing of STM as well as SE strains. A questionnaire held among the NRLs revealed that for comparison between laboratories, standardisation of the antimicrobial susceptibility testing would have to be a requirement.